Literature DB >> 4077843

A streamlined method of subfragment one preparation from myosin.

Y Okamoto, T Sekine.   

Abstract

A rapid procedure for isolating subfragment one (SF1) from myosin was found. SF1 can be isolated specifically from proteolytic digests of myosin in the presence of a millimolar concentration of magnesium chloride. Under such ionic conditions all of the rod portion and undigested myosin is selectively precipitated. A nucleotide trapping experiment indicated how important quick preparation of SF1 is for maintaining the active site structure. This method can also be utilized in the preparation of heavy meromyosin.

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Year:  1985        PMID： 4077843     DOI： 10.1093/oxfordjournals.jbchem.a135365

Source DB:  PubMed          Journal:  J Biochem        ISSN： 0021-924X            Impact factor:   3.387


  16 in total

1.  Linear dichroism of acrylodan-labeled tropomyosin and myosin subfragment 1 bound to actin in myofibrils.

Authors:  D Szczesna; S S Lehrer
Journal:  Biophys J       Date:  1992-04       Impact factor: 4.033

2.  Phalloidin affects the myosin-dependent sliding velocities of actin filaments in a bound-divalent cation dependent manner.

Authors:  K Tokuraku; T Q Uyeda
Journal:  J Muscle Res Cell Motil       Date:  2001       Impact factor: 2.698

3.  Myosin cleft closure determines the energetics of the actomyosin interaction.

Authors:  Balázs Takács; Elizabeth O'Neall-Hennessey; Csaba Hetényi; József Kardos; Andrew G Szent-Györgyi; Mihály Kovács
Journal:  FASEB J       Date:  2010-09-13       Impact factor: 5.191

4.  Orientation of the myosin light chain region by single molecule total internal reflection fluorescence polarization microscopy.

Authors:  Margot E Quinlan; Joseph N Forkey; Yale E Goldman
Journal:  Biophys J       Date:  2005-05-13       Impact factor: 4.033

5.  Longitudinal distortions and transversal fluctuations of an actin filament sliding on Myosin molecules.

Authors:  H Honda; K Kikuchi; K Hatori; E Imai; K Shimada; K Matsuno
Journal:  J Biol Phys       Date:  2002-09       Impact factor: 1.365

6.  Yeast actin filaments display ATP-dependent sliding movement over surfaces coated with rabbit muscle myosin.

Authors:  S J Kron; D G Drubin; D Botstein; J A Spudich
Journal:  Proc Natl Acad Sci U S A       Date:  1992-05-15       Impact factor: 11.205

7.  Nucleotide trapping at the ATPase site of myosin subfragment 1 by a new interthiol crosslinking.

Authors:  P Chaussepied; D Mornet; R Kassab
Journal:  Proc Natl Acad Sci U S A       Date:  1986-04       Impact factor: 11.205

8.  Nucleotide specificity of the enzymatic and motile activities of dynein, kinesin, and heavy meromyosin.

Authors:  T Shimizu; K Furusawa; S Ohashi; Y Y Toyoshima; M Okuno; F Malik; R D Vale
Journal:  J Cell Biol       Date:  1991-03       Impact factor: 10.539

9.  Regulation of binding of subfragment 1 in isolated rigor myofibrils.

Authors:  D R Swartz; M L Greaser; B B Marsh
Journal:  J Cell Biol       Date:  1990-12       Impact factor: 10.539

10.  Molecular Mechanisms of the Deregulation of Muscle Contraction Induced by the R90P Mutation in Tpm3.12 and the Weakening of This Effect by BDM and W7.

Authors:  Yurii S Borovikov; Daria D Andreeva; Stanislava V Avrova; Vladimir V Sirenko; Armen O Simonyan; Charles S Redwood; Olga E Karpicheva
Journal:  Int J Mol Sci       Date:  2021-06-12       Impact factor: 5.923
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