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Literature DB >> 2938184

Nucleotide trapping at the ATPase site of myosin subfragment 1 by a new interthiol crosslinking.

Abstract

When myosin subfragment 1 derivatives in which the reactive sulfhydryl SH1 has been blocked react with N,N'-p-phenylenedimaleimide or 5,5'-dithiobis(2-nitrobenzoic acid), the reactive sulfhydryl group SH2 of the 20-kDa domain is crosslinked with a thiol of the 50-kDa domain of the heavy chain. The crosslink induces the stable trapping of a significant amount of Mg2+-nucleotide in the ATPase site.

Entities: Chemical Gene

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Year: 1986 PMID： 2938184 PMCID： PMC323225 DOI： 10.1073/pnas.83.7.2037

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

30 in total

1. Structure of the actin-myosin interface.

Authors: D Mornet; R Bertrand; P Pantel; E Audemard; R Kassab
Journal: Nature Date: 1981-07-23 Impact factor: 49.962

2. Proteolysis and the domain organization of myosin subfragment 1.

Authors: D Mornet; K Ue; M F Morales
Journal: Proc Natl Acad Sci U S A Date: 1984-02 Impact factor: 11.205

3. Sulfhydryl modification and labeling of myosin.

Authors: E Reisler
Journal: Methods Enzymol Date: 1982 Impact factor: 1.600

4. Spectroscopic isolation of ES complexes of myosin subfragment-1 ATPase by fluorescence quenching.

Authors: T Ando; J A Duke; Y Tonomura; M F Morales
Journal: Biochem Biophys Res Commun Date: 1982-11-16 Impact factor: 3.575

5. Interactions of ATP analogues, N6-[(6-aminohexyl) Carbamoylmethyl] ATP and its dinitrophenyl derivative, with the active site of myosin.

Authors: K Yamamoto; T Sekine
Journal: J Biochem Date: 1982-11 Impact factor: 3.387

6. On the mechanism of energy transduction in myosin subfragment 1.

Authors: J Botts; R Takashi; P Torgerson; T Hozumi; A Muhlrad; D Mornet; M F Morales
Journal: Proc Natl Acad Sci U S A Date: 1984-04 Impact factor: 11.205

7. F-actin is intermolecularly crosslinked by N,N'-p-phenylenedimaleimide through lysine-191 and cysteine-374.

Authors: M Elzinga; J J Phelan
Journal: Proc Natl Acad Sci U S A Date: 1984-11 Impact factor: 11.205

8. Crosslinked myosin subfragment 1: a stable analogue of the subfragment-1.ATP complex.

Authors: J M Chalovich; L E Greene; E Eisenberg
Journal: Proc Natl Acad Sci U S A Date: 1983-08 Impact factor: 11.205

9. Trapping of transition metal-nucleotide complexes in myosin subfragment 1 by cross-linking thiols; divalent transition metal probes of the active site.

Authors: R E Dalbey; J A Wells; R G Yount
Journal: Biochemistry Date: 1983-01-18 Impact factor: 3.162

10. Photochemical probes of the active site of myosin. Irradiation of trapped 3'-O-(4-benzoyl)benzoyladenosine 5'-triphosphate labels the 50-kilodalton heavy chain tryptic peptide.

Authors: R Mahmood; R G Yount
Journal: J Biol Chem Date: 1984-11-10 Impact factor: 5.157

6 in total

1. Actin and temperature effects on the cross-linking of the SH1-SH2 helix in myosin subfragment 1.

Authors: L K Nitao; E Reisler
Journal: Biophys J Date: 2000-06 Impact factor: 4.033

Review 2. Domains, motions and regulation in the myosin head.

Authors: P Vibert; C Cohen
Journal: J Muscle Res Cell Motil Date: 1988-08 Impact factor: 2.698

3. Modifying preselected sites on proteins: the stretch of residues 633-642 of the myosin heavy chain is part of the actin-binding site.

Authors: P Chaussepied; M F Morales
Journal: Proc Natl Acad Sci U S A Date: 1988-10 Impact factor: 11.205