| Literature DB >> 4075282 |
M Ochiai, M Nagao, T Tahira, F Ishikawa, K Hayashi, H Ohgaki, M Terada, N Tsuchida, T Sugimura.
Abstract
Oncogenes of fibrosarcomas of rats, induced by subcutaneous injection of 1,8-dinitropyrene (1,8-DNP), were examined by NIH 3T3 cell transfection assay and Southern blot analysis. Transformants containing rat specific repetitive sequences were obtained with DNAs of 4 fibrosarcomas, 1,8-DNP1, 1,8-DNP2, 1,8-DNP3 and 1,8-DNP7. A transformant, 1,8-DNP2-2, induced by DNA of a fibrosarcoma, 1,8-DNP2, and 7 secondary transformants derived from it contained rat K-ras sequences. Another transformant, 1,8-DNP2-1, induced by the same sarcoma did not have a ras family oncogene. This indicates that the sarcoma, 1,8-DNP2, has at least 2 transforming genes. The transforming genes of 6 other transformants derived from 3 sarcomas did not contain ras family or neu transforming genes.Entities:
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Year: 1985 PMID： 4075282 DOI： 10.1016/0304-3835(85)90150-8
Source DB: PubMed Journal: Cancer Lett ISSN： 0304-3835 Impact factor: 8.679