| Literature DB >> 4066036 |
A C Wright, J G Morris, D R Maneval, K Richardson, J B Kaper.
Abstract
Genes encoding the cytotoxin-hemolysin of Vibrio vulnificus were cloned in Escherichia coli by using the lytic cloning vector, lambda 1059. Subcloning in plasmid pBR325 resulted in the isolation of a 3.2-kilobase DNA fragment containing the cytotoxin gene. By using this fragment as a DNA probe, homologous gene sequences were detected in all 54 V. vulnificus strains studied; homologous sequences were present in none of 96 isolates from 29 other bacterial species.Entities:
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Year: 1985 PMID: 4066036 PMCID: PMC261170 DOI: 10.1128/iai.50.3.922-924.1985
Source DB: PubMed Journal: Infect Immun ISSN: 0019-9567 Impact factor: 3.441