Literature DB >> 17277209

Multiplex PCR assay for detection of Vibrio vulnificus biotype 2 and simultaneous discrimination of serovar E strains.

Eva Sanjuán1, Carmen Amaro.   

Abstract

In the present work we develop a multiplex PCR assay for the detection and identification of the fish pathogen Vibrio vulnificus biotype 2 with discriminating potential for zoonotic strains (serovar E). The PCR assay allowed the identification of two new biotype 2 serovar E human isolates from culture collections. Finally, the multiplex was successfully applied to both diagnosis and carrier detection in field samples.

Entities:  

Mesh：

Substances：

Year:  2007        PMID： 17277209      PMCID： PMC1828805          DOI： 10.1128/AEM.02320-06

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  22 in total

1.  The cytolysin gene of Vibrio vulnificus: sequence and relationship to the Vibrio cholerae E1 Tor hemolysin gene.

Authors:  K Yamamoto; A C Wright; J B Kaper; J G Morris
Journal:  Infect Immun       Date:  1990-08       Impact factor: 3.441

2.  Clinical, epidemiological, and microbiological features of Vibrio vulnificus biogroup 3 causing outbreaks of wound infection and bacteraemia in Israel. Israel Vibrio Study Group.

Authors:  N Bisharat; V Agmon; R Finkelstein; R Raz; G Ben-Dror; L Lerner; S Soboh; R Colodner; D N Cameron; D L Wykstra; D L Swerdlow; J J Farmer
Journal:  Lancet       Date:  1999-10-23       Impact factor: 79.321

3.  Clinical manifestations and molecular epidemiology of Vibrio vulnificus infections in Denmark.

Authors:  A Dalsgaard; N Frimodt-Møller; B Bruun; L Høi; J L Larsen
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1996-03       Impact factor: 3.267

Review 4.  Pathogenesis of Vibrio vulnificus.

Authors:  D A Linkous; J D Oliver
Journal:  FEMS Microbiol Lett       Date:  1999-05-15       Impact factor: 2.742

5.  [Vibrio vulnificus infections in Denmark during the summer of 1994].

Authors:  B G Bruun; N Frimodt-Møller; A Dalsgaard; H E Busk; H Friis; H J Kolmos; E Laursen; J Prag; N Rosdahl; P Schouenborg; P Søgaard
Journal:  Ugeskr Laeger       Date:  1996-07-22

6.  Vibrio vulnificus biotype 2, pathogenic for eels, is also an opportunistic pathogen for humans.

Authors:  C Amaro; E G Biosca
Journal:  Appl Environ Microbiol       Date:  1996-04       Impact factor: 4.792

7.  Protocol for specific isolation of virulent strains of Vibrio vulnificus serovar E (biotype 2) from environmental samples.

Authors:  Eva Sanjuán; Carmen Amaro
Journal:  Appl Environ Microbiol       Date:  2004-12       Impact factor: 4.792

8.  Cloning of the cytotoxin-hemolysin gene of Vibrio vulnificus.

Authors:  A C Wright; J G Morris; D R Maneval; K Richardson; J B Kaper
Journal:  Infect Immun       Date:  1985-12       Impact factor: 3.441

9.  Simultaneous detection of Aeromonas salmonicida, Flavobacterium psychrophilum, and Yersinia ruckeri, three major fish pathogens, by multiplex PCR.

Authors:  A Del Cerro; I Marquez; J A Guijarro
Journal:  Appl Environ Microbiol       Date:  2002-10       Impact factor: 4.792

10.  The first fatal case of Vibrio vulnificus infection in Denmark.

Authors:  T Bock; N Christensen; N H Eriksen; S Winter; H Rygaard; F Jørgensen
Journal:  APMIS       Date:  1994-11       Impact factor: 3.205
View more
  10 in total

1.  Sialic acid catabolism and transport gene clusters are lineage specific in Vibrio vulnificus.

Authors:  Jean-Bernard Lubin; Joseph J Kingston; Nityananda Chowdhury; E Fidelma Boyd
Journal:  Appl Environ Microbiol       Date:  2012-02-17       Impact factor: 4.792

2.  Evaluation of genotypic and phenotypic methods to distinguish clinical from environmental Vibrio vulnificus strains.

Authors:  Eva Sanjuán; Belén Fouz; James D Oliver; Carmen Amaro
Journal:  Appl Environ Microbiol       Date:  2009-01-09       Impact factor: 4.792

3.  Prevalence and population structure of Vibrio vulnificus on fishes from the northern Gulf of Mexico.

Authors:  Zhen Tao; Andrea M Larsen; Stephen A Bullard; Anita C Wright; Covadonga R Arias
Journal:  Appl Environ Microbiol       Date:  2012-08-24       Impact factor: 4.792

4.  Vibrio vulnificus biotype 2 serovar E gne but not galE is essential for lipopolysaccharide biosynthesis and virulence.

Authors:  Esmeralda Valiente; Natalia Jiménez; Susana Merino; Juan M Tomás; Carmen Amaro
Journal:  Infect Immun       Date:  2008-01-28       Impact factor: 3.441

5.  pilF Polymorphism-based PCR to distinguish vibrio vulnificus strains potentially dangerous to public health.

Authors:  Francisco J Roig; Eva Sanjuán; Amparo Llorens; Carmen Amaro
Journal:  Appl Environ Microbiol       Date:  2009-12-28       Impact factor: 4.792

6.  Virulence Profiles of Vibrio vulnificus in German Coastal Waters, a Comparison of North Sea and Baltic Sea Isolates.

Authors:  Nadja Bier; Claudia Jäckel; Ralf Dieckmann; Nicole Brennholt; Simone I Böer; Eckhard Strauch
Journal:  Int J Environ Res Public Health       Date:  2015-12-15       Impact factor: 3.390

7.  Molecular typing of environmental and clinical strains of Vibrio vulnificus isolated in the northeastern USA.

Authors:  Yann Reynaud; Steven Pitchford; Sophie De Decker; Gary H Wikfors; Christopher L Brown
Journal:  PLoS One       Date:  2013-12-30       Impact factor: 3.240

8.  Occurrence of Virulence Genes Associated with Human Pathogenic Vibrios Isolated from Two Commercial Dusky Kob (Argyrosmus japonicus) Farms and Kareiga Estuary in the Eastern Cape Province, South Africa.

Authors:  Justine Fri; Roland Ndip Ndip; Henry Akum Njom; Anna Maria Clarke
Journal:  Int J Environ Res Public Health       Date:  2017-09-25       Impact factor: 3.390

9.  The widespread presence of a family of fish virulence plasmids in Vibrio vulnificus stresses its relevance as a zoonotic pathogen linked to fish farms.

Authors:  Héctor Carmona-Salido; Belén Fouz; Eva Sanjuán; Miguel Carda; Christian M J Delannoy; Neris García-González; Fernando González-Candelas; Carmen Amaro
Journal:  Emerg Microbes Infect       Date:  2021-12       Impact factor: 7.163

10.  Wild eel microbiome reveals that skin mucus of fish could be a natural niche for aquatic mucosal pathogen evolution.

Authors:  Miguel Carda-Diéguez; Rohit Ghai; Francisco Rodríguez-Valera; Carmen Amaro
Journal:  Microbiome       Date:  2017-12-21       Impact factor: 14.650
  10 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.