| Literature DB >> 4062287 |
L A Tabak, L Mirels, L D Monte, A L Ridall, M J Levine, R E Loomis, F Lindauer, M S Reddy, B J Baum.
Abstract
A blood group A+ mucin-glycoprotein was purified from aqueous extracts of rat submandibular glands by sequential chromatography on columns of Sepharose CL-6B and Sephacryl S-300 in urea-containing buffers. Final purification was facilitated by reductive methylation which appeared to release contaminating (hydrophobic) peptides. Homogeneity of the purified mucin was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis at varying concentrations of acrylamide, lectin affinity chromatography, and Western blot analysis. In contrast to previously described preparations, the purified mucin contained only trace amounts of N-acetylglucosamine and aromatic amino acids. In addition, only low levels of basic amino acids were present.Entities:
Mesh：
Substances：
Year: 1985 PMID： 4062287 DOI： 10.1016/0003-9861(85)90222-x
Source DB: PubMed Journal: Arch Biochem Biophys ISSN： 0003-9861 Impact factor: 4.013