Literature DB >> 4044530

Polyethylene glycol-facilitated transformation of Bacteroides fragilis with plasmid DNA.

C J Smith.   

Abstract

A method for the transformation of Bacteroides fragilis with plasmid DNA was developed by using the clindamycin resistance plasmid pBFTM10 as the source of transforming DNA. The method was technically simple to perform and resulted in an average of 4.2 X 10(3) transformants per microgram of pBFTM10 added. A method for the preparation of frozen competent cells is also described.

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Year:  1985        PMID： 4044530      PMCID： PMC214267          DOI： 10.1128/jb.164.1.466-469.1985

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  15 in total

1.  Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid.

Authors:  E M Lederberg; S N Cohen
Journal:  J Bacteriol       Date:  1974-09       Impact factor: 3.490

2.  Effect of diet on human fecal flora: comparison of Japanese and American diets.

Authors:  S M Finegold; H R Attebery; V L Sutter
Journal:  Am J Clin Nutr       Date:  1974-12       Impact factor: 7.045

3.  Calcium-dependent bacteriophage DNA infection.

Authors:  M Mandel; A Higa
Journal:  J Mol Biol       Date:  1970-10-14       Impact factor: 5.469

4.  Characterization of pBFTM10, a clindamycin-erythromycin resistance transfer factor from Bacteroides fragilis.

Authors:  F P Tally; D R Snydman; M J Shimell; M H Malamy
Journal:  J Bacteriol       Date:  1982-08       Impact factor: 3.490

5.  High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA.

Authors:  S Chang; S N Cohen
Journal:  Mol Gen Genet       Date:  1979-01-05

6.  Transfer of multiple antibiotic resistance between subspecies of Bacteroides fragilis.

Authors:  G Privitera; A Dublanchet; M Sebald
Journal:  J Infect Dis       Date:  1979-01       Impact factor: 5.226

7.  Two independent conjugal transfer systems operating in Bacteroides fragilis V479-1.

Authors:  C J Smith; R A Welch; F L Macrina
Journal:  J Bacteriol       Date:  1982-07       Impact factor: 3.490

8.  Genetic transformation of Rhizobium meliloti by plasmid DNA.

Authors:  G Selvaraj; V N Iyer
Journal:  Gene       Date:  1981-11       Impact factor: 3.688

9.  Transformation of Rhizobium meliloti 41 with plasmid DNA.

Authors:  G B Kiss; Z Kálmán
Journal:  J Bacteriol       Date:  1982-05       Impact factor: 3.490

10.  Human fecal flora: the normal flora of 20 Japanese-Hawaiians.

Authors:  W E Moore; L V Holdeman
Journal:  Appl Microbiol       Date:  1974-05
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  9 in total

1.  Nucleotide sequence analysis of Tn4551: use of ermFS operon fusions to detect promoter activity in Bacteroides fragilis.

Authors:  C J Smith
Journal:  J Bacteriol       Date:  1987-10       Impact factor: 3.490

2.  Mini review: Molecular genetics: A new tool for investigating the microbial ecology of the gastrointestinal tract?

Authors:  G W Tannock
Journal:  Microb Ecol       Date:  1988-05       Impact factor: 4.552

3.  Conjugal transfer of transposon Tn916 from Streptococcus faecalis to Mycoplasma hominis.

Authors:  M C Roberts; G E Kenny
Journal:  J Bacteriol       Date:  1987-08       Impact factor: 3.490

4.  Transposition of Tn4551 in Bacteroides fragilis: identification and properties of a new transposon from Bacteroides spp.

Authors:  C J Smith; H Spiegel
Journal:  J Bacteriol       Date:  1987-08       Impact factor: 3.490

5.  Complete nucleotide sequence and transcription of ermF, a macrolide-lincosamide-streptogramin B resistance determinant from Bacteroides fragilis.

Authors:  J L Rasmussen; D A Odelson; F L Macrina
Journal:  J Bacteriol       Date:  1986-11       Impact factor: 3.490

6.  Use of targeted insertional mutagenesis to determine whether chondroitin lyase II is essential for chondroitin sulfate utilization by Bacteroides thetaiotaomicron.

Authors:  E P Guthrie; A A Salyers
Journal:  J Bacteriol       Date:  1986-06       Impact factor: 3.490

7.  Isolation and characterization of a temperate bacteriophage from the ruminal anaerobe Selenomonas ruminantium.

Authors:  R A Lockington; G T Attwood; J D Brooker
Journal:  Appl Environ Microbiol       Date:  1988-06       Impact factor: 4.792

8.  Regions in Bacteroides plasmids pBFTM10 and pB8-51 that allow Escherichia coli-Bacteroides shuttle vectors to be mobilized by IncP plasmids and by a conjugative Bacteroides tetracycline resistance element.

Authors:  N B Shoemaker; C Getty; E P Guthrie; A A Salyers
Journal:  J Bacteriol       Date:  1986-06       Impact factor: 3.490

9.  Development and use of cloning systems for Bacteroides fragilis: cloning of a plasmid-encoded clindamycin resistance determinant.

Authors:  C J Smith
Journal:  J Bacteriol       Date:  1985-10       Impact factor: 3.490
  9 in total

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