Literature DB >> 4038876

A simple method for purification of rat hepatic microsomal cytochrome b5.

A Burchell.   

Abstract

Rat hepatic microsomal cytochrome b5 was purified to homogeneity by solubilization with the detergent Lubrol 12-A9 and chromatography on Fractogel TSK DEAE-650(S). The protein was obtained in high yield (52-87%) in 8 h, and only one polypeptide band, of Mr 16 600, was visible after sodium dodecyl sulphate/polyacrylamide-gel electrophoresis.

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Year:  1985        PMID： 4038876      PMCID： PMC1144712          DOI： 10.1042/bj2260339

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  19 in total

1.  The roles of cytochrome b5 in a reconstituted N-demethylase system containing cytochrome P-450.

Authors:  Y Imai; R Sato
Journal:  Biochem Biophys Res Commun       Date:  1977-03-21       Impact factor: 3.575

2.  The isolation and properties of microsomal cytochrome.

Authors:  P STRITTMATTER; S F VELICK
Journal:  J Biol Chem       Date:  1956-07       Impact factor: 5.157

3.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

4.  Rat liver microsomal elongation of fatty acids. Possible involvement of cytochrome b5.

Authors:  S R Keyes; J A Alfano; I Jansson; D L Cinti
Journal:  J Biol Chem       Date:  1979-08-25       Impact factor: 5.157

5.  Cytochrome b5 from microsomal membranes of equine, bovine, and porcine livers. Isolation and properties of preparations containing the membranous segment.

Authors:  J Ozols
Journal:  Biochemistry       Date:  1974-01-29       Impact factor: 3.162

6.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

7.  The measurement of difference spectra: application to the cytochromes of microsomes.

Authors:  R W Estabrook; J Werringloer
Journal:  Methods Enzymol       Date:  1978       Impact factor: 1.600

8.  Evidence for the participation of cytochrome b5 in plasmalogen biosynthesis.

Authors:  F Paltuaf; R A Prough; B S Masters; J M Johnston
Journal:  J Biol Chem       Date:  1974-04-25       Impact factor: 5.157

9.  A form of cytochrome b5 that contains an additional hydrophobic sequence of 40 amino acid residues.

Authors:  L Spatz; P Strittmatter
Journal:  Proc Natl Acad Sci U S A       Date:  1971-05       Impact factor: 11.205

10.  Mechanism of C-5 double bond introduction in the biosynthesis of cholesterol by rat liver microsomes.

Authors:  V V Reddy; D Kupfer; E Caspi
Journal:  J Biol Chem       Date:  1977-05-10       Impact factor: 5.157
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  4 in total

1.  Export of a hyperexpressed mammalian globular cytochrome b5 precursor in Escherichia coli is dramatically affected by the nature of the amino acid flanking the secretory signal sequence cleavage bond.

Authors:  Naheed N Kaderbhai; Khalil Ahmed; Mustak A Kaderbhai
Journal:  Protein Sci       Date:  2010-07       Impact factor: 6.725

2.  Stabilization of glucose-6-phosphatase activity by a 21 000-dalton hepatic microsomal protein.

Authors:  A Burchell; B Burchell; M Monaco; H E Walls; W J Arion
Journal:  Biochem J       Date:  1985-09-01       Impact factor: 3.857

3.  OnpA, an unusual flavin-dependent monooxygenase containing a cytochrome b(5) domain.

Authors:  Yi Xiao; Ting-Ting Liu; Hui Dai; Jun-Jie Zhang; Hong Liu; Huiru Tang; David J Leak; Ning-Yi Zhou
Journal:  J Bacteriol       Date:  2012-01-20       Impact factor: 3.490

4.  Rat hepatic microsomal cytochrome b5. A simple large-scale purification procedure and antibody production by antigen-containing liposomes.

Authors:  J Carlsen; K Christiansen; H M Jensen
Journal:  Biochem J       Date:  1988-12-15       Impact factor: 3.857
  4 in total

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