Literature DB >> 3223944

Rat hepatic microsomal cytochrome b5. A simple large-scale purification procedure and antibody production by antigen-containing liposomes.

J Carlsen1, K Christiansen, H M Jensen.   

Abstract

Cytochrome b5 from rat liver microsomes (microsomal fractions) was purified in its native form. The procedure described has great capacity, is fast, and the final product is pure as judged from SDS/polyacrylamide-gel electrophoresis. Antibodies to cytochrome b5 are obtained after administration of the antigen inserted into small unilamellar lipid vesicles.

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Year:  1988        PMID： 3223944      PMCID： PMC1135524          DOI： 10.1042/bj2561051

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  13 in total

1.  THE INTERACTION OF PORPHYRINS AND METALLOPORHYRINS WITH APOCYTOCHROME BETA-5.

Authors:  J OZOLS; P STRITTMATTER
Journal:  J Biol Chem       Date:  1964-04       Impact factor: 5.157

2.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

3.  Water-soluble and pentanol-extractable proteins in human brain normal tissue and human brain tumours, with special reference to S-100 protein.

Authors:  K G Haglid; D Stavrou
Journal:  J Neurochem       Date:  1973-06       Impact factor: 5.372

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Liposomes as a tool to study the role of membrane presentation in the immunogenicity of a MuLV-related tumor antigen.

Authors:  D Gerlier; O Bakouche; J F Dore
Journal:  J Immunol       Date:  1983-07       Impact factor: 5.422

6.  Studies on the biosynthesis of microsomal membrane proteins. Site of synthesis and mode of insertion of cytochrome b5, cytochrome b5 reductase, cytochrome P-450 reductase and epoxide hydrolase.

Authors:  Y Okada; A B Frey; T M Guenthner; F Oesch; D D Sabatini; G Kreibich
Journal:  Eur J Biochem       Date:  1982-02

7.  Chemical structure of rat liver cytochrome b5. Isolation of peptides by high-pressure liquid chromatography.

Authors:  J Ozols; F S Heinemann
Journal:  Biochim Biophys Acta       Date:  1982-05-21

8.  Immunization, isolation of immunoglobulins, estimation of antibody titre.

Authors:  N Harboe; A Ingild
Journal:  Scand J Immunol Suppl       Date:  1973

9.  The membrane-embedded segment of cytochrome b5 as studied by cross-linking with photoactivatable phospholipids. II. The nontransferable form.

Authors:  Y Takagaki; R Radhakrishnan; K W Wirtz; H G Khorana
Journal:  J Biol Chem       Date:  1983-08-10       Impact factor: 5.157

10.  A simple method for purification of rat hepatic microsomal cytochrome b5.

Authors:  A Burchell
Journal:  Biochem J       Date:  1985-02-15       Impact factor: 3.857
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