Literature DB >> 4037305

Generating sucrose gradients in three minutes by tilted tube rotation.

D H Coombs, N R Watts.   

Abstract

A technique which permits rapid preparation of sucrose gradients with highly reproducible profiles is described. Tubes are filled with equal volumes of light and heavy sucrose solutions, sealed, and rotated for 3 min tilted 80 degrees from vertical. Linear 5-20% and 5-30% gradients and nonlinear but useful 5-45% gradients are obtained.

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Year:  1985        PMID： 4037305     DOI： 10.1016/0003-2697(85)90654-2

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  10 in total

1.  Structure of the bacteriophage T4 baseplate as determined by chemical cross-linking.

Authors:  N R Watts; D H Coombs
Journal:  J Virol       Date:  1990-01       Impact factor: 5.103

2.  Gradient reconstitution of membrane proteins for solid-state NMR studies.

Authors:  Denis Lacabanne; Alons Lends; Clément Danis; Britta Kunert; Marie-Laure Fogeron; Vlastimil Jirasko; Claire Chuilon; Lauriane Lecoq; Cédric Orelle; Vincent Chaptal; Pierre Falson; Jean-Michel Jault; Beat H Meier; Anja Böckmann
Journal:  J Biomol NMR       Date:  2017-09-12       Impact factor: 2.835

3.  The Rab27a/granuphilin complex regulates the exocytosis of insulin-containing dense-core granules.

Authors:  Zhaohong Yi; Hiromi Yokota; Seiji Torii; Takeo Aoki; Masahiro Hosaka; Shengli Zhao; Kuniaki Takata; Toshiyuki Takeuchi; Tetsuro Izumi
Journal:  Mol Cell Biol       Date:  2002-03       Impact factor: 4.272

4.  Two forms of aryl hydrocarbon receptor type 2 in rainbow trout (Oncorhynchus mykiss). Evidence for differential expression and enhancer specificity.

Authors:  C C Abnet; R L Tanguay; M E Hahn; W Heideman; R E Peterson
Journal:  J Biol Chem       Date:  1999-05-21       Impact factor: 5.157

5.  Glyceraldehyde-3-phosphate dehydrogenase activity and F-actin associations in synaptosomes and postsynaptic densities of porcine cerebral cortex.

Authors:  A A Rogalski-Wilk; R S Cohen
Journal:  Cell Mol Neurobiol       Date:  1997-02       Impact factor: 5.046

6.  Analysis of near-neighbor contacts in bacteriophage T4 wedges and hubless baseplates by using a cleavable chemical cross-linker.

Authors:  N R Watts; D H Coombs
Journal:  J Virol       Date:  1989-06       Impact factor: 5.103

7.  An immunoblot assay reveals that bacteriophage T4 thymidylate synthase and dihydrofolate reductase are not virion proteins.

Authors:  X Chen; C K Mathews; L J Wheeler; G Maley; F Maley; D H Coombs
Journal:  J Virol       Date:  1995-04       Impact factor: 5.103

8.  GRecon: a method for the lipid reconstitution of membrane proteins.

Authors:  Thorsten Althoff; Karen M Davies; Sabrina Schulze; Friederike Joos; Werner Kühlbrandt
Journal:  Angew Chem Int Ed Engl       Date:  2012-07-23       Impact factor: 15.336

9.  Arrangement of electron transport chain components in bovine mitochondrial supercomplex I1III2IV1.

Authors:  Thorsten Althoff; Deryck J Mills; Jean-Luc Popot; Werner Kühlbrandt
Journal:  EMBO J       Date:  2011-09-09       Impact factor: 11.598

10.  A pseudoatomic model of the COPII cage obtained from cryo-electron microscopy and mass spectrometry.

Authors:  Alex J Noble; Qian Zhang; Jason O'Donnell; Hanaa Hariri; Nilakshee Bhattacharya; Alan G Marshall; Scott M Stagg
Journal:  Nat Struct Mol Biol       Date:  2012-12-23       Impact factor: 15.369
  10 in total

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