Literature DB >> 4030697

Role of protein subunits in Proteus rettgeri penicillin G acylase.

G O Daumy, D Danley, A S McColl.   

Abstract

Penicillin G acylase from Proteus rettgeri is an 80,000- to 90,000-dalton enzyme composed of two nonidentical subunits. Both subunits were required for enzymatic activity. The 65,000-dalton beta subunit contained a phenylmethylsulfonyl fluoride-sensitive residue required for enzymatic activity, and the 24,500-dalton alpha subunit contained the domain that imparts specificity for the penicillin side chain.

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Year:  1985        PMID： 4030697      PMCID： PMC219274          DOI： 10.1128/jb.163.3.1279-1281.1985

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  7 in total

1.  DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.

Authors:  B J DAVIS
Journal:  Ann N Y Acad Sci       Date:  1964-12-28       Impact factor: 5.691

2.  Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.

Authors:  D W Cleveland; S G Fischer; M W Kirschner; U K Laemmli
Journal:  J Biol Chem       Date:  1977-02-10       Impact factor: 5.157

3.  Preparation and general properties of crystalline penicillin acylase from Escherichia coli ATCC 11 105.

Authors:  C Kutzbach; E Rauenbusch
Journal:  Hoppe Seylers Z Physiol Chem       Date:  1974-01

4.  Spectrophotometric determination of microgram quantities of protein without nucleic acid interference.

Authors:  W E Groves; F C Davis; B H Sells
Journal:  Anal Biochem       Date:  1968-02       Impact factor: 3.365

5.  Experimental evolution of penicillin G acylases from Escherichia coli and Proteus rettgeri.

Authors:  G O Daumy; D Danley; A S McColl; D Apostolakos; F J Vinick
Journal:  J Bacteriol       Date:  1985-09       Impact factor: 3.490

6.  Role of primary structure and disulfide bond formation in beta-lactamase secretion.

Authors:  S Pollitt; H Zalkin
Journal:  J Bacteriol       Date:  1983-01       Impact factor: 3.490

7.  Latent proteinase activity of gamma-glutamyl transpeptidase light subunit.

Authors:  S J Gardell; S S Tate
Journal:  J Biol Chem       Date:  1979-06-25       Impact factor: 5.157
  7 in total
  12 in total

1.  Processing and functional display of the 86 kDa heterodimeric penicillin G acylase on the surface of phage fd.

Authors:  R M Verhaert; J Van Duin; W J Quax
Journal:  Biochem J       Date:  1999-09-01       Impact factor: 3.857

2.  Chemical modification of serine at the active site of penicillin acylase from Kluyvera citrophila.

Authors:  J Martín; A Slade; A Aitken; R Arche; R Virden
Journal:  Biochem J       Date:  1991-12-15       Impact factor: 3.857

3.  Molecular biology of β-lactam acylases.

Authors:  B S Deshpande; S S Ambedkar; V K Sudhakaran; J G Shewale
Journal:  World J Microbiol Biotechnol       Date:  1994-03       Impact factor: 3.312

4.  Crystal structure of penicillin G acylase from the Bro1 mutant strain of Providencia rettgeri.

Authors:  M A McDonough; H E Klei; J A Kelly
Journal:  Protein Sci       Date:  1999-10       Impact factor: 6.725

5.  Molecular cloning and analysis of the gene encoding the thermostable penicillin G acylase from Alcaligenes faecalis.

Authors:  R M Verhaert; A M Riemens; J M van der Laan; J van Duin; W J Quax
Journal:  Appl Environ Microbiol       Date:  1997-09       Impact factor: 4.792

6.  Two-step autocatalytic processing of the glutaryl 7-aminocephalosporanic acid acylase from Pseudomonas sp. strain GK16.

Authors:  Y S Lee; S S Park
Journal:  J Bacteriol       Date:  1998-09       Impact factor: 3.490

7.  Inactivation of penicillin acylase from Kluyvera citrophila by N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline: a case of time-dependent non-covalent enzyme inhibition.

Authors:  J Martín; J M Mancheño; R Arche
Journal:  Biochem J       Date:  1993-05-01       Impact factor: 3.857

8.  Expression and regulation of the penicillin G acylase gene from Proteus rettgeri cloned in Escherichia coli.

Authors:  G O Daumy; J A Williams; A S McColl; T J Zuzel; D Danley
Journal:  J Bacteriol       Date:  1986-10       Impact factor: 3.490

9.  Molecular cloning of the penicillin G acylase gene from Arthrobacter viscosus.

Authors:  H Ohashi; Y Katsuta; T Hashizume; S N Abe; H Kajiura; H Hattori; T Kamei; M Yano
Journal:  Appl Environ Microbiol       Date:  1988-11       Impact factor: 4.792

10.  Changing the substrate specificity of penicillin G acylase from Kluyvera citrophila through selective pressure.

Authors:  A Roa; J L Garcia; F Salto; E Cortes
Journal:  Biochem J       Date:  1994-11-01       Impact factor: 3.857
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