Prolactin permits the expression of a circadian variation in insulin receptor profile in hepatocytes of the golden hamster (Mesocricetus auratus).

Insulin receptor profile was determined in freshly isolated hamster hepatocytes at two times (07.00 and 16.00 h) during a 14-h daily photoperiod (08.00-22.00 h). The hamsters were pretreated for 5 days with bromocriptine (to inhibit prolactin release), bromocriptine and prolactin replacement, or control saline injections. The insulin receptor profile was determined by Scatchard plot analysis. The insulin receptor number (high and low affinity) was three times greater at 07.00 than at 16.00 h among saline-injected controls. However, their affinities did not differ. Bromocriptine pretreatment reduced (70%) both the high and low affinity receptor numbers and increased the affinity of the high affinity receptor at 07.00 h. Prolactin replacement in bromocriptine-treated hamsters restored the receptor profile at 07.00 h to control values. These data indicate that prolactin facilitates the expression of a circadian variation in insulin receptor profile.