Literature DB >> 4015617

Two-dimensional gel electrophoresis of proteins for genetic studies in Douglas fir (Pseudotsuga menziesii).

N Bahrman, D de Vienne, H Thiellement, J P Hofmann.   

Abstract

A method of two-dimensional gel electrophoresis of proteins from Douglas fir needles is described. Extraction in the presence of sodium dodecyl sulfate (SDS) and 2-mercaptoethanol followed by heating at 100 degrees C produces reliable two-dimensional gels which are convenient for genetic studies. Three genotypes from different geographical origins have been compared: among 225 loci expressed, 22 display regulatory variations and 7 show allelic variations. Thus it is now possible to undertake the genetic study of Douglas fir using this powerful technique.

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Year:  1985        PMID： 4015617     DOI： 10.1007/bf00504322

Source DB:  PubMed          Journal:  Biochem Genet        ISSN： 0006-2928            Impact factor:   1.890


  9 in total

1.  Two-Dimensional Electrophoretic Mapping of Proteins of Bundle Sheath and Mesophyll Cells of the C(4) Grass Digitaria sanguinalis (L.) Scop. (Crabgrass).

Authors:  P A Harrison; C C Black
Journal:  Plant Physiol       Date:  1982-11       Impact factor: 8.340

2.  Analysis of Leaf Proteins by Two-Dimensional Gel Electrophoresis: Protease Action as Exemplified by Ribulose Bisphosphate Carboxylase/ Oxygenase Degradation and Procedure to Avoid Proteolysis during Extraction.

Authors:  C C des Francs; H Thiellement; D de Vienne
Journal:  Plant Physiol       Date:  1985-05       Impact factor: 8.340

3.  High resolution two-dimensional electrophoresis of proteins.

Authors:  P H O'Farrell
Journal:  J Biol Chem       Date:  1975-05-25       Impact factor: 5.157

4.  A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels.

Authors:  B R Oakley; D R Kirsch; N R Morris
Journal:  Anal Biochem       Date:  1980-07-01       Impact factor: 3.365

5.  Two-dimensional gel electrophoresis of membrane proteins.

Authors:  G F Ames; K Nikaido
Journal:  Biochemistry       Date:  1976-02-10       Impact factor: 3.162

6.  Genetic variability of soluble proteins studied by two-dimensional electrophoresis on different inbred mouse strains and on different mouse organs.

Authors:  J Klose
Journal:  J Mol Evol       Date:  1982       Impact factor: 2.395

7.  Study on nuclear and cytoplasmic genome expression in wheat by two-dimensional gel electrophoresis : 2. Genetic differences between two lines and two groups of cytoplasms at five developmental stages or organs.

Authors:  M Zivy; H Thiellement; D de Vienne; J P Hofmann
Journal:  Theor Appl Genet       Date:  1984-07       Impact factor: 5.699

8.  Two-dimensional polyacylamide gel electrophoresis of envelope proteins of Escherichia coli.

Authors:  W C Johnson; T J Silhavy; W Boos
Journal:  Appl Microbiol       Date:  1975-03

9.  Study on nuclear and cytoplasmic genome expression in wheat by two-dimensional gel electrophoresis : 1. First results on 18 alloplasmic lines.

Authors:  M Zivy; H Thiellement; D de Vienne; J P Hofmann
Journal:  Theor Appl Genet       Date:  1983-07       Impact factor: 5.699
  9 in total
  5 in total

Review 1.  Large-scale plant proteomics.

Authors:  Birgit Kersten; Lukas Bürkle; Eckehard J Kuhn; Patrick Giavalisco; Zoltan Konthur; Angelika Lueking; Gerald Walter; Holger Eickhoff; Ulrich Schneider
Journal:  Plant Mol Biol       Date:  2002-01       Impact factor: 4.076

2.  Regulatory effects of homoeologous chromosome arms on wheat proteins at two developmental stages.

Authors:  H Thiellement; N Bahrman; C Colas des Francs
Journal:  Theor Appl Genet       Date:  1986-12       Impact factor: 5.699

3.  Chromosomal localization of structural genes and regulators in wheat by 2D electrophoresis of ditelosomic lines.

Authors:  C Colas des Francs; H Thiellement
Journal:  Theor Appl Genet       Date:  1985-11       Impact factor: 5.699

4.  Is the polymorphism of protein amounts related to phenotypic variability? A comparison of two-dimensional electrophoresis data with morphological traits in maize.

Authors:  C Damerval; Y Hébert; D de Vienne
Journal:  Theor Appl Genet       Date:  1987-06       Impact factor: 5.699

5.  Association of protein amount polymorphism (PAP) among maize lines with performances of their hybrids.

Authors:  A Leonardi; C Damerval; Y Hébert; A Gallais; D de Vienne
Journal:  Theor Appl Genet       Date:  1991-10       Impact factor: 5.699
  5 in total

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