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Literature DB >> 16664194

Analysis of Leaf Proteins by Two-Dimensional Gel Electrophoresis: Protease Action as Exemplified by Ribulose Bisphosphate Carboxylase/ Oxygenase Degradation and Procedure to Avoid Proteolysis during Extraction.

C C des Francs¹, H Thiellement, D de Vienne.

Abstract

A previous genetic analysis by two-dimensional electrophoresis of wheat leaf proteins led us to hypothesize that several polypeptides were degradation products of the large subunit of ribulose bisphosphate carboxylase/oxygenase (EC 4.1.1.39).VARIOUS EXTRACTION PROCEDURES INCLUDING THE USE OF PROTEINASE INHIBITORS ALLOWED US TO: (a) confirm that the suspected polypeptides are indeed degradation products; (b) find out under which conditions the proteinases act; (c) find a method which prevents degradation, by boiling the extract in the presence of sodium dodecyl sulfate and 2-mercaptoethanol.

Entities: Species

Year: 1985 PMID： 16664194 PMCID： PMC1064698 DOI： 10.1104/pp.78.1.178

Source DB: PubMed Journal: Plant Physiol ISSN： 0032-0889 Impact factor: 8.340

9 in total

1. Two-Dimensional Electrophoretic Mapping of Proteins of Bundle Sheath and Mesophyll Cells of the C(4) Grass Digitaria sanguinalis (L.) Scop. (Crabgrass).

Authors: P A Harrison; C C Black
Journal: Plant Physiol Date: 1982-11 Impact factor: 8.340

2. Partial purification and characterization of endoproteinases from senescing barley leaves.

Authors: B L Miller; R C Huffaker
Journal: Plant Physiol Date: 1981-10 Impact factor: 8.340

3. High resolution two-dimensional electrophoresis of proteins.

Authors: P H O'Farrell
Journal: J Biol Chem Date: 1975-05-25 Impact factor: 5.157

4. A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels.

Authors: B R Oakley; D R Kirsch; N R Morris
Journal: Anal Biochem Date: 1980-07-01 Impact factor: 3.365

5. Ribulose Bisphosphate Carboxylase and Proteolytic Activity in Wheat Leaves from Anthesis through Senescence.

Authors: V A Wittenbach
Journal: Plant Physiol Date: 1979-11 Impact factor: 8.340

6. Proteases of Senescing Oat Leaves: II. Reaction to Substrates and Inhibitors.

Authors: R H Drivdahl; K V Thimann
Journal: Plant Physiol Date: 1978-04 Impact factor: 8.340

7. Breakdown of Ribulose Bisphosphate Carboxylase and Change in Proteolytic Activity during Dark-induced Senescence of Wheat Seedlings.

Authors: V A Wittenbach
Journal: Plant Physiol Date: 1978-10 Impact factor: 8.340

8. Source of endoproteolytic activity associated with purified ribulose bisphosphate carboxylase.

Authors: J L Rosichan; R C Huffaker
Journal: Plant Physiol Date: 1984-05 Impact factor: 8.340

9. Studies on human plasma alpha 2-macroglobulin-enzyme interactions. Evidence for proteolytic modification of the subunit chain structure.

Authors: P C Harpel
Journal: J Exp Med Date: 1973-09-01 Impact factor: 14.307

9 in total

20 in total

1. Differential Expression of Proteins and mRNAs from Border Cells and Root Tips of Pea.

Authors: L. A. Brigham; H. H. Woo; S. M. Nicoll; M. C. Hawes
Journal: Plant Physiol Date: 1995-10 Impact factor: 8.340

2. Solubilization of plant membrane proteins for analysis by two-dimensional gel electrophoresis.

Authors: W J Hurkman; C K Tanaka
Journal: Plant Physiol Date: 1986-07 Impact factor: 8.340

3. Regulatory effects of homoeologous chromosome arms on wheat proteins at two developmental stages.

Authors: H Thiellement; N Bahrman; C Colas des Francs
Journal: Theor Appl Genet Date: 1986-12 Impact factor: 5.699

4. Chromosomal localization of structural genes and regulators in wheat by 2D electrophoresis of ditelosomic lines.

Authors: C Colas des Francs; H Thiellement
Journal: Theor Appl Genet Date: 1985-11 Impact factor: 5.699

5. Is the polymorphism of protein amounts related to phenotypic variability? A comparison of two-dimensional electrophoresis data with morphological traits in maize.

Authors: C Damerval; Y Hébert; D de Vienne
Journal: Theor Appl Genet Date: 1987-06 Impact factor: 5.699

6. Proteases of Melilotus alba mesophyll protoplasts : II. General properties and effectiveness in degradation of cytosolic and vacuolar enzymes.

Authors: H Canut; M Dupré; A Carrasco; A M Boudet
Journal: Planta Date: 1987-04 Impact factor: 4.116