Literature DB >> 4014658

Microquantitative analysis of neutral and amino sugars as fluorescent pyridylamino derivatives by high-performance liquid chromatography.

H Takemoto, S Hase, T Ikenaka.   

Abstract

A method for the quantitation of picomole amounts of neutral and amino sugars in glycoconjugates was developed. Glycoconjugates were hydrolyzed with a mixture of equal amounts of 4 M trifluoroacetic acid and 4 M hydrochloric acid, and the free amino groups were acetylated. Sugars were coupled with 2-aminopyridine. After the excess reagents were removed by gel-permeation high-performance liquid chromatography, the fluorescent pyridylamino derivatives of sugars were separated and quantified by high-performance liquid chromatography on a reversed-phase column. This method allowed the determination of 0.01-10 nmol of sugars. About 100 pmol of several glycoconjugates were analyzed by the present method, with satisfactory results.

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Year:  1985        PMID： 4014658     DOI： 10.1016/0003-2697(85)90357-4

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  8 in total

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Authors:  S J Kim; M Shoda
Journal:  Appl Environ Microbiol       Date:  1999-03       Impact factor: 4.792

2.  Characterization of mucin in whole-gut lavage fluid obtained from patients with inflammatory bowel disease.

Authors:  H Saitoh; K Takagaki; T Nakamura; A Munakata; Y Yoshida; M Endo
Journal:  Dig Dis Sci       Date:  1996-09       Impact factor: 3.199

3.  Possible role of hepatic bile mucus glycoprotein in development of intrahepatic gallstones.

Authors:  S Akaishi; S Yoshihara; M Sasaki; M Konn
Journal:  J Gastroenterol       Date:  1995-12       Impact factor: 7.527

4.  Histochemical analysis of blood group antigens in human sublingual glands and pancreas. An application of high-performance liquid chromatography to estimate the quantity of galactose liberated from tissue sections by alpha-galactosidase digestion.

Authors:  N Ito; S Tabata; S Kawahara; Y Hirano; K Nakajima; K Uchida; T Hirota
Journal:  Histochem J       Date:  1993-03

5.  Platelet-interactive products of Streptococcus sanguis protoplasts.

Authors:  M C Herzberg; P R Erickson; P K Kane; D J Clawson; C C Clawson; F A Hoff
Journal:  Infect Immun       Date:  1990-12       Impact factor: 3.441

6.  Carbohydrate moieties of porcine 32 kDa enamelin.

Authors:  Y Yamakoshi
Journal:  Calcif Tissue Int       Date:  1995-04       Impact factor: 4.333

7.  Vibrio cholerae O1 strain TSI-4 produces the exopolysaccharide materials that determine colony morphology, stress resistance, and biofilm formation.

Authors:  S N Wai; Y Mizunoe; A Takade; S I Kawabata; S I Yoshida
Journal:  Appl Environ Microbiol       Date:  1998-10       Impact factor: 4.792

Review 8.  Pyridylamination as a means of analyzing complex sugar chains.

Authors:  Sumihiro Hase
Journal:  Proc Jpn Acad Ser B Phys Biol Sci       Date:  2010       Impact factor: 3.493
  8 in total

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