Literature DB >> 4000926

Methylation of thymine residues during oligonucleotide synthesis.

X Gao, B L Gaffney, M Senior, R R Riddle, R A Jones.   

Abstract

Thymine residues in an oligodeoxyribonucleotide are subject to methylation at N3 by the internucleotide methyl phosphotriester linkages. This alkylation occurs most rapidly in the presence of a strong base such as DBU, but also takes place, at a much slower rate, during oligonucleotide synthesis.

Entities:  

Mesh：

Substances：

Year:  1985        PMID： 4000926      PMCID： PMC341015          DOI： 10.1093/nar/13.2.573

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  9 in total

1.  Large scale chemical synthesis, purification and crystallization of RNA-DNA chimeras.

Authors:  N Usman; M Egli; A Rich
Journal:  Nucleic Acids Res       Date:  1992-12-25       Impact factor: 16.971

2.  Specificity and efficiency of editing of mismatches involved in the formation of base-substitution mutations by the 3'----5' exonuclease activity of phage T4 DNA polymerase.

Authors:  N K Sinha
Journal:  Proc Natl Acad Sci U S A       Date:  1987-02       Impact factor: 11.205

3.  RNA-mediated gene assembly from DNA arrays.

Authors:  Cheng-Hsien Wu; Matthew R Lockett; Lloyd M Smith
Journal:  Angew Chem Int Ed Engl       Date:  2012-03-30       Impact factor: 15.336

4.  Solid supported hydrolysis of apurinic sites in synthetic oligonucleotides for rapid and efficient purification on reverse-phase cartridges.

Authors:  T Horn; M S Urdea
Journal:  Nucleic Acids Res       Date:  1988-12-23       Impact factor: 16.971

5.  Modification of guanine bases by nucleoside phosphoramidite reagents during the solid phase synthesis of oligonucleotides.

Authors:  R T Pon; M J Damha; K K Ogilvie
Journal:  Nucleic Acids Res       Date:  1985-09-25       Impact factor: 16.971

6.  Application of 2-cyanoethyl N,N,N',N'-tetraisopropylphosphorodiamidite for in situ preparation of deoxyribonucleoside phosphoramidites and their use in polymer-supported synthesis of oligodeoxyribonucleotides.

Authors:  J Nielsen; M Taagaard; J E Marugg; J H van Boom; O Dahl
Journal:  Nucleic Acids Res       Date:  1986-09-25       Impact factor: 16.971

7.  Synthesis and structure assignments of amide protected nucleosides and their use as phosphoramidites in deoxyoligonucleotide synthesis.

Authors:  M Mag; J W Engels
Journal:  Nucleic Acids Res       Date:  1988-04-25       Impact factor: 16.971

8.  Highly efficient oligodeoxyribonucleotide synthesis using fully base protected phosphodiester building blocks carrying 2-(1-methylimidazol-2-yl) phenyl protection of the phosphate.

Authors:  B S Sproat; P Rider; B Beijer
Journal:  Nucleic Acids Res       Date:  1986-02-25       Impact factor: 16.971

9.  Modifications of guanine bases during oligonucleotide synthesis.

Authors:  A T Yeung; W J Dinehart; B K Jones
Journal:  Nucleic Acids Res       Date:  1988-05-25       Impact factor: 16.971
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.