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Literature DB >> 3972456

Selective detection of Mycoplasma hyorhinis using cloned genomic DNA fragments.

Abstract

A 13-kilobase DNA fragment from a genomic library of Mycoplasma hyorhinis demonstrated specific Southern hybridization and dot hybridization when tested against a group of different mycoplasmas. This probe selectively recognized M. hyorhinis sequences in purified DNA, broth-grown organisms, and infected cell cultures, providing a direct method for differential detection of this species.

Entities: Disease Species

Mesh：

Substances：
DNA, Bacterial

Year: 1985 PMID： 3972456 PMCID： PMC261402 DOI： 10.1128/iai.47.3.827-830.1985

Source DB: PubMed Journal: Infect Immun ISSN： 0019-9567 Impact factor: 3.441

17 in total

1. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors: P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal: J Mol Biol Date: 1977-06-15 Impact factor: 5.469

2. Selective association of murine T lymphoblastoid cell surface alloantigens with Mycoplasma hyorhinis.

Authors: K S Wise; G H Cassell; R T Action
Journal: Proc Natl Acad Sci U S A Date: 1978-09 Impact factor: 11.205

3. A simple method to recover intact high molecular weight RNA and DNA after electrophoretic separation in low gelling temperature agarose gels.

Authors: L Wieslander
Journal: Anal Biochem Date: 1979-10-01 Impact factor: 3.365

Selective detection of Mycoplasma hyorhinis using cloned genomic DNA fragments.

1. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

2. Selective association of murine T lymphoblastoid cell surface alloantigens with Mycoplasma hyorhinis.

3. A simple method to recover intact high molecular weight RNA and DNA after electrophoretic separation in low gelling temperature agarose gels.

4. Genetic Differentiation by Nucleic Acid Homology II. Genotypic Variations Within Two Mycoplasma Species.

5. Genetic differentiation by nucleic Acid homology I. Relationships among Mycoplasma species of man.

6. Physical mapping of the ribosomal RNA genes of Mycoplasma capricolum.

7. Detection of mycoplasmas infecting cell cultures by DNA hybridization.

8. Ribosomal RNA genes in Mycoplasma.

9. Mycoplasma hyorhinis GDL surface protein antigen p120 defined by monoclonal antibody.

10. Cloned genomic DNA sequences from Mycoplasma hyorhinis encoding antigens expressed in Escherichia coli.

1. Detection and quantitation of Anaplasma marginale in carrier cattle by using a nucleic acid probe.

2. Lipid-modified surface protein antigens expressing size variation within the species Mycoplasma hyorhinis.

3. A species-specific DNA probe for the detection of Mycoplasma gallisepticum.

4. DNA probes for clinical applications. Patents and literature.

Review 5. Molecular biology and genetics of mycoplasmas (Mollicutes).

6. Major membrane surface proteins of Mycoplasma hyopneumoniae selectively modified by covalently bound lipid.

7. A cloned DNA probe identifies Cowdria ruminantium in Amblyomma variegatum ticks.

8. An amplifiable DNA region from the Mycoplasma hyorhinis genome.