| Literature DB >> 3967763 |
H Bloemendal, M Willemsen, G Groenewoud, P Oomen.
Abstract
A novel, simple and relatively rapid method is described for the isolation of the intermediate-sized filament protein vimentin from eye lens tissue. Chromatofocusing is applied as the sole purification step. The apparent isoelectric point of the protein in 6 M urea and at 22 degrees C is 4.9. Electrophoretic mobility on one- and two-dimensional polyacrylamide gels, solubility in 6 M urea and amino acid composition were used for identification.Entities:
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Year: 1985 PMID： 3967763 DOI： 10.1016/0014-5793(85)81067-x
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124