Transcriptional and post-transcriptional inhibition of albumin gene expression by estrogen in Xenopus liver.

The purpose of this study was to investigate the suppression of albumin mRNA by estrogen in Xenopus liver. A single dose of estradiol rapidly suppressed albumin mRNA to 30% of the control level. Albumin mRNA remained at this new steady-state level for 9 days, after which it returned to the control level. Transcription 'run-on' experiments in isolated liver nuclei demonstrated a transient decrease of 60-90% in albumin transcription after 2-6 h and approached constitutive transcription by 12 h. Albumin gene transcription then remained constant for the following 12 days. Prolonged and enhanced suppression of albumin mRNA was observed in animals treated repeatedly with estrogen for 12 days. In these animals, albumin gene transcription was decreased 80-90% from the constitutive control level. These data indicate that albumin mRNA is suppressed by both transcriptional and post-transcriptional mechanisms.