Literature DB >> 3955180

The intrinsic pKa values for phosphatidylserine and phosphatidylethanolamine in phosphatidylcholine host bilayers.

F C Tsui, D M Ojcius, W L Hubbell.   

Abstract

Potentiometric titrations and surface potential measurements have been used to determine the intrinsic pKa values of both the carboxyl and amino groups of phosphatidylserine (PS) in mixed vesicles of PS and phosphatidylcholine (PC), and also of the amino group of phosphatidylethanolamine (PE) in mixed PE-PC vesicles. The pKa of the carboxyl group of PS in liposomes with different PS/PC lipid ratios measured by the two different methods is 3.6 +/- 0.1, and the pKa of its amino group is 9.8 +/- 0.1. The pKa of the amino group of PE in PE-PC vesicles, determined solely by surface potential measurements, is 9.6 +/- 0.1. These pKa values are independent of the aqueous phase ionic strength and of the effect of the liposome's surface potential due to the presence of these partially charged lipids.

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Year:  1986        PMID： 3955180      PMCID： PMC1329485          DOI： 10.1016/S0006-3495(86)83655-4

Source DB:  PubMed          Journal:  Biophys J        ISSN： 0006-3495            Impact factor:   4.033


  21 in total

1.  Ionic influences on the phase transition of dipalmitoylphosphatidylserine.

Authors:  R C MacDonald; S A Simon; E Baer
Journal:  Biochemistry       Date:  1976-02-24       Impact factor: 3.162

2.  Adsorption of monovalent cations to bilayer membranes containing negative phospholipids.

Authors:  M Eisenberg; T Gresalfi; T Riccio; S McLaughlin
Journal:  Biochemistry       Date:  1979-11-13       Impact factor: 3.162

3.  Estimation of membrane surface potential and charge density from the phase equilibrium of a paramagnetic amphiphile.

Authors:  J D Castle; W L Hubbell
Journal:  Biochemistry       Date:  1976-11-02       Impact factor: 3.162

4.  Comparative studies on the effects of pH and Ca2+ on bilayers of various negatively charged phospholipids and their mixtures with phosphatidylcholine.

Authors:  P W van Dijck; B de Kruijff; A J Verkleij; L L van Deenen; J de Gier
Journal:  Biochim Biophys Acta       Date:  1978-09-11

5.  Lamellar structure of bleached and unbleached rod photoreceptor membranes.

Authors:  J M Corless
Journal:  Nature       Date:  1972-05-26       Impact factor: 49.962

6.  The effect of charge and cholesterol on the size and thickness of sonicated phospholipid vesicles.

Authors:  S M Johnson
Journal:  Biochim Biophys Acta       Date:  1973-04-25

7.  EPR determination of membrane potentials.

Authors:  D S Cafiso; W L Hubbell
Journal:  Annu Rev Biophys Bioeng       Date:  1981

8.  Perturbations of membrane structure by optical probes: I. Location and structural sensitivity of merocyanine 540 bound to phospholipid membranes.

Authors:  P I Lelkes; I R Miller
Journal:  J Membr Biol       Date:  1980-01-31       Impact factor: 1.843

9.  The stacking of chloroplast thylakoids. Quantitative analysis of the balance of forces between thylakoid membranes of chloroplasts, and the role of divalent cations.

Authors:  M J Sculley; J T Duniec; S W Thorne; W S Chow; N K Boardman
Journal:  Arch Biochem Biophys       Date:  1980-04-15       Impact factor: 4.013

10.  Titration of the phase transition of phosphatidylserine bilayer membranes. Effects of pH, surface electrostatics, ion binding, and head-group hydration.

Authors:  G Cevc; A Watts; D Marsh
Journal:  Biochemistry       Date:  1981-08-18       Impact factor: 3.162
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  69 in total

1.  Electrostatic properties of fiber cell membranes from the frog lens.

Authors:  L R Pasquale; R T Mathias; L R Austin; P R Brink; M Ciunga
Journal:  Biophys J       Date:  1990-10       Impact factor: 4.033

Review 2.  Lipophilicity and its relationship with passive drug permeation.

Authors:  Xiangli Liu; Bernard Testa; Alfred Fahr
Journal:  Pharm Res       Date:  2010-10-30       Impact factor: 4.200

3.  Conformational states and dynamics of rhodopsin in micelles and bilayers.

Authors:  Ana Karin Kusnetzow; Christian Altenbach; Wayne L Hubbell
Journal:  Biochemistry       Date:  2006-05-02       Impact factor: 3.162

4.  Tetanus toxin channel in phosphatidylserine planar bilayers: conductance states and pH dependence.

Authors:  G Rauch; F Gambale; M Montal
Journal:  Eur Biophys J       Date:  1990       Impact factor: 1.733

5.  A novel method for assessment of local pH in periplasmic space and of cell surface potential in yeast.

Authors:  Jaromír Plášek; David Babuka; Dana Gášková; Iva Jančíková; Jakub Zahumenský; Milan Hoefer
Journal:  J Bioenerg Biomembr       Date:  2017-04-12       Impact factor: 2.945

6.  Shapes and coiling of mixed phospholipid vesicles.

Authors:  Gerardo Paredes-Quijada; Helim Aranda-Espinoza; Amir Maldonado
Journal:  Lipids       Date:  2009-01-27       Impact factor: 1.880

7.  Quantitative fluorescence microscopy using supported lipid bilayer standards.

Authors:  William J Galush; Jeffrey A Nye; Jay T Groves
Journal:  Biophys J       Date:  2008-05-30       Impact factor: 4.033

8.  Protons migrate along interfacial water without significant contributions from jumps between ionizable groups on the membrane surface.

Authors:  Andreas Springer; Volker Hagen; Dmitry A Cherepanov; Yuri N Antonenko; Peter Pohl
Journal:  Proc Natl Acad Sci U S A       Date:  2011-08-22       Impact factor: 11.205

9.  HIV gp41 six-helix bundle constructs induce rapid vesicle fusion at pH 3.5 and little fusion at pH 7.0: understanding pH dependence of protein aggregation, membrane binding, and electrostatics, and implications for HIV-host cell fusion.

Authors:  Kelly Sackett; Allan TerBush; David P Weliky
Journal:  Eur Biophys J       Date:  2011-01-11       Impact factor: 1.733

10.  Distribution of charge on photoreceptor disc membranes and implications for charged lipid asymmetry.

Authors:  F C Tsui; S A Sundberg; W L Hubbell
Journal:  Biophys J       Date:  1990-01       Impact factor: 4.033
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