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Literature DB >> 3951994

Differential effects of dimethylsulfoxide on the activities of human DNA polymerases alpha and delta.

Abstract

The effects of dimethylsulfoxide on the activities of purified human placental DNA polymerase alpha and DNA polymerase delta were examined. DNA polymerase alpha was inhibited by dimethylsulfoxide, whereas DNA polymerase delta was significantly activated, by as much as 6-fold. Kinetic data show that the effect of dimethylsulfoxide on DNA polymerase delta activity was due to a reduction in the apparent Km for its substrate, dTTP. This novel finding of the differential effects of dimethylsulfoxide on the activities of polymerases alpha and delta may be useful in their identification and differential assay.

Entities: Chemical Gene Species

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Year: 1986 PMID： 3951994 PMCID： PMC339568 DOI： 10.1093/nar/14.4.1719

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

16 in total

1. The effect of DMSO on several enzyme systems.

Authors: D H Rammler
Journal: Ann N Y Acad Sci Date: 1967-03-15 Impact factor: 5.691

2. Hemoglobin synthesis in murine virus-induced leukemic cells in vitro: stimulation of erythroid differentiation by dimethyl sulfoxide.

Authors: C Friend; W Scher; J G Holland; T Sato
Journal: Proc Natl Acad Sci U S A Date: 1971-02 Impact factor: 11.205

3. Differential inhibition of human placental DNA polymerases delta and alpha by BuPdGTP and BuAdATP.

Authors: M Y Lee; N L Toomey; G E Wright
Journal: Nucleic Acids Res Date: 1985-12-09 Impact factor: 16.971

Review 4. Replication of eukaryotic chromosomes: a close-up of the replication fork.

Authors: M L DePamphilis; P M Wassarman
Journal: Annu Rev Biochem Date: 1980 Impact factor: 23.643

5. Intracellular localization of human DNA polymerase alpha with monoclonal antibodies.

Authors: K G Bensch; S Tanaka; S Z Hu; T S Wang; D Korn
Journal: J Biol Chem Date: 1982-07-25 Impact factor: 5.157

6. Further studies on calf thymus DNA polymerase delta purified to homogeneity by a new procedure.

Authors: M Y Lee; C K Tan; K M Downey; A G So
Journal: Biochemistry Date: 1984-04-24 Impact factor: 3.162

7. Purification of deoxyribonucleic acid polymerase delta from calf thymus: partial characterization of physical properties.

Authors: M Y Lee; C K Tan; A G So; K M Downey
Journal: Biochemistry Date: 1980-05-13 Impact factor: 3.162

Differential effects of dimethylsulfoxide on the activities of human DNA polymerases alpha and delta.

1. The effect of DMSO on several enzyme systems.

2. Hemoglobin synthesis in murine virus-induced leukemic cells in vitro: stimulation of erythroid differentiation by dimethyl sulfoxide.

3. Differential inhibition of human placental DNA polymerases delta and alpha by BuPdGTP and BuAdATP.

Review 4. Replication of eukaryotic chromosomes: a close-up of the replication fork.

5. Intracellular localization of human DNA polymerase alpha with monoclonal antibodies.

6. Further studies on calf thymus DNA polymerase delta purified to homogeneity by a new procedure.

7. Purification of deoxyribonucleic acid polymerase delta from calf thymus: partial characterization of physical properties.

8. DNA-dependent RNA polymerase from the extremely halophilic archaebacterium Halococcus morrhuae.

9. Stimulation of tyrosine protein kinase activity by dimethyl sulfoxide.

10. Monoclonal antibodies against human DNA polymerase-alpha inhibit DNA replication in permeabilized human cells.

1. Molecular cloning of the cDNA for the catalytic subunit of human DNA polymerase delta.

2. Minichromosome assembly accompanying repair-type DNA synthesis in Xenopus oocytes.

3. DNA polymerases alpha, delta, and epsilon: three distinct enzymes from HeLa cells.