Literature DB >> 1542570

Molecular cloning of the cDNA for the catalytic subunit of human DNA polymerase delta.

C L Yang1, L S Chang, P Zhang, H Hao, L Zhu, N L Toomey, M Y Lee.   

Abstract

The cDNA of human DNA polymerase delta was cloned. The cDNA had a length of 3.5 kb and encoded a protein of 1107 amino acid residues with a calculated molecular mass of 124 kDa. Northern blot analysis showed that the cDNA hybridized to a mRNA of 3.4 kb. Monoclonal and polyclonal antibodies to the C-terminal 20 residues specifically immunoblotted the human pol delta catalytic polypeptide. A multiple sequence alignment was constructed. This showed that human pol delta is closely related to yeast pol delta and the herpes virus DNA polymerases. The levels of pol delta message were found to be induced concomitantly with DNA pol delta activity and DNA synthesis in serum restimulated proliferating IMR90 cultured cells. The human pol delta gene was localized to chromosome 19 by Southern blotting of EcoRI digested DNA from a panel of rodent/human cell hybrids.

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Year:  1992        PMID： 1542570      PMCID： PMC312012          DOI： 10.1093/nar/20.4.735

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  69 in total

1.  Biochemical characterization and development of DNA polymerases alpha and delta in the neonatal rat heart.

Authors:  S J Zhang; M Y Lee
Journal:  Arch Biochem Biophys       Date:  1987-01       Impact factor: 4.013

2.  Gene expression of human DNA polymerase alpha during cell proliferation and the cell cycle.

Authors:  A F Wahl; A M Geis; B H Spain; S W Wong; D Korn; T S Wang
Journal:  Mol Cell Biol       Date:  1988-11       Impact factor: 4.272

Review 3.  An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs.

Authors:  M Kozak
Journal:  Nucleic Acids Res       Date:  1987-10-26       Impact factor: 16.971

4.  Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.

Authors:  S Henikoff
Journal:  Gene       Date:  1984-06       Impact factor: 3.688

5.  Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements.

Authors:  J J Dunn; F W Studier
Journal:  J Mol Biol       Date:  1983-06-05       Impact factor: 5.469

6.  Purification of deoxyribonucleic acid polymerase delta from calf thymus: partial characterization of physical properties.

Authors:  M Y Lee; C K Tan; A G So; K M Downey
Journal:  Biochemistry       Date:  1980-05-13       Impact factor: 3.162

7.  DNA polymerase II is encoded by the DNA damage-inducible dinA gene of Escherichia coli.

Authors:  C A Bonner; S Hays; K McEntee; M F Goodman
Journal:  Proc Natl Acad Sci U S A       Date:  1990-10       Impact factor: 11.205

8.  Purification and characterization of two new high molecular weight forms of DNA polymerase delta.

Authors:  J J Crute; A F Wahl; R A Bambara
Journal:  Biochemistry       Date:  1986-01-14       Impact factor: 3.162

9.  Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold.

Authors:  J E Walker; M Saraste; M J Runswick; N J Gay
Journal:  EMBO J       Date:  1982       Impact factor: 11.598

10.  Human DNA polymerase alpha gene expression is cell proliferation dependent and its primary structure is similar to both prokaryotic and eukaryotic replicative DNA polymerases.

Authors:  S W Wong; A F Wahl; P M Yuan; N Arai; B E Pearson; K Arai; D Korn; M W Hunkapiller; T S Wang
Journal:  EMBO J       Date:  1988-01       Impact factor: 11.598
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  17 in total

1.  DNA polymerase delta isolated from Schizosaccharomyces pombe contains five subunits.

Authors:  S Zuo; E Gibbs; Z Kelman; T S Wang; M O'Donnell; S A MacNeill; J Hurwitz
Journal:  Proc Natl Acad Sci U S A       Date:  1997-10-14       Impact factor: 11.205

2.  The p12 subunit of human polymerase delta modulates the rate and fidelity of DNA synthesis.

Authors:  Xiao Meng; Yajing Zhou; Ernest Y C Lee; Marietta Y W T Lee; David N Frick
Journal:  Biochemistry       Date:  2010-05-04       Impact factor: 3.162

3.  New nucleotide sequence data on the EMBL File Server.

Authors: 
Journal:  Nucleic Acids Res       Date:  1992-05-11       Impact factor: 16.971

4.  E2F mediates induction of the Sp1-controlled promoter of the human DNA polymerase epsilon B-subunit gene POLE2.

Authors:  D Huang; M Jokela; J Tuusa; S Skog; K Poikonen; J E Syväoja
Journal:  Nucleic Acids Res       Date:  2001-07-01       Impact factor: 16.971

5.  Structural and functional relationships of human DNA polymerases.

Authors:  H Hao; Y Jiang; S J Zhang; P Zhang; R X Zeng; M Y Lee
Journal:  Chromosoma       Date:  1992       Impact factor: 4.316

6.  Complementation of defective translesion synthesis and UV light sensitivity in xeroderma pigmentosum variant cells by human and mouse DNA polymerase eta.

Authors:  A Yamada; C Masutani; S Iwai; F Hanaoka
Journal:  Nucleic Acids Res       Date:  2000-07-01       Impact factor: 16.971

7.  Human DNA polymerase epsilon is expressed during cell proliferation in a manner characteristic of replicative DNA polymerases.

Authors:  J Tuusa; L Uitto; J E Syväoja
Journal:  Nucleic Acids Res       Date:  1995-06-25       Impact factor: 16.971

8.  The 3'-->5' exonuclease associated with HeLa DNA polymerase epsilon.

Authors:  L Uitto; J Halleen; P Remes; T Kesti; J E Syväoja
Journal:  Chromosoma       Date:  1992       Impact factor: 4.316

9.  The 3'-5' exonuclease of human DNA polymerase delta (pol delta) is regulated by pol delta accessory factors and deoxyribonucleoside triphosphates.

Authors:  S H Lee
Journal:  Nucleic Acids Res       Date:  1993-04-25       Impact factor: 16.971

10.  Organization and nucleotide sequence of the DNA polymerase gene from the archaeon Pyrococcus furiosus.

Authors:  T Uemori; Y Ishino; H Toh; K Asada; I Kato
Journal:  Nucleic Acids Res       Date:  1993-01-25       Impact factor: 16.971
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