Alignment maps and homology analysis of the J-C intron in human, mouse, and rabbit immunoglobulin kappa gene.

The statistically significant shared oligonucleotides (block identities) of the intervening region (J5-C) in the human, mouse, and rabbit immunoglobulin (Ig)-kappa gene were determined. These identities maintain their order (do not cross) and never connect with any Ig-kappa segment external to the intron region. The two regions of pronounced similarity are (1) the vicinity of the established enhancer element (Queen and Baltimore 1983) and (2) a 200-bp region approximately 1 kb upstream that we have labeled the second enhancer element. Similarity is strong between the human and mouse sequences in the neighborhood of the first enhancer element and more pronounced between the human and rabbit sequences in the vicinity of the second enhancer region. The overall extent of similarity between the mouse and rabbit sequences is less than that between the human and mouse sequences and that between the human and rabbit sequences. All close and large dyad-symmetry pairings were ascertained and their possible relations to the enhancer elements discussed.