Literature DB >> 3937955

Purification of the human anionic polypeptide fraction of the apo-bile lipoprotein complex by zonal ultracentrifugation.

M Martigne, N Domingo, H Lafont, G Nalbone, J C Hauton.   

Abstract

The two main proteic constituents of the human Apo-bile lipoprotein complex (BLC), i.e., the anionic polypeptide fraction (APF) and the IgA fragments, were separated by preparative zonal ultracentrifugation using a sucrose gradient containing 1.5 mM glycodesoxycholate. The purification of the APF was verified by sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis and immunology, and its amino acid composition then was determined. This procedure was used to obtain a polyclonal antiserum directed solely against the APF.

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Year:  1985        PMID： 3937955     DOI： 10.1007/bf02534772

Source DB:  PubMed          Journal:  Lipids        ISSN： 0024-4201            Impact factor:   1.880


  22 in total

1.  Immunological relationship between bile lipoprotein complex and high density lipoprotein.

Authors:  J L Vigne; H Lafont; G Nalbone; N Domingo; M Charbonnier-Augeire; D Lairon; J Léonardi; J C Hauton
Journal:  Biochem Biophys Res Commun       Date:  1979-06-27       Impact factor: 3.575

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Journal:  Clin Chim Acta       Date:  1972-08       Impact factor: 3.786

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Authors:  J C Montet; D G Dervichian
Journal:  Biochimie       Date:  1971       Impact factor: 4.079

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Authors:  C Henry; D Bernard; R Depieds
Journal:  Ann Inst Pasteur (Paris)       Date:  1968-03

5.  The equilibrium and structure of lecithin-cholate mixed micelles.

Authors:  W Shankland
Journal:  Chem Phys Lipids       Date:  1970-04       Impact factor: 3.329

6.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

7.  Solubilisation of cholesterol in human bile.

Authors:  N R Pattinson
Journal:  FEBS Lett       Date:  1985-02-25       Impact factor: 4.124

8.  Taurocholate- and taurochenodeoxycholate-lecithin micelles: the equilibrium of bile salt between aqueous phase and micelle.

Authors:  W C Duane
Journal:  Biochem Biophys Res Commun       Date:  1977-01-10       Impact factor: 3.575

9.  Biliary proteins. Unique inhibitors of cholesterol crystal nucleation in human gallbladder bile.

Authors:  R T Holzbach; A Kibe; E Thiel; J H Howell; M Marsh; R E Hermann
Journal:  J Clin Invest       Date:  1984-01       Impact factor: 14.808

10.  Zone electrophoresis study of the bile lipoprotein complex.

Authors:  H Lafont; G Nalbone; D Lairon; J C Dagorn; N Domingo; J Amic; J C Hauton
Journal:  Biochimie       Date:  1977       Impact factor: 4.079
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  3 in total

1.  Isolation of an acidic protein from cholesterol gallstones, which inhibits the precipitation of calcium carbonate in vitro.

Authors:  S Shimizu; B Sabsay; A Veis; J D Ostrow; R V Rege; L G Dawes
Journal:  J Clin Invest       Date:  1989-12       Impact factor: 14.808

2.  Comparative effects on biliary concanavalin A-bound glycoproteins and calcium ion on cholesterol crystal nucleation and growth in model bile.

Authors:  K Teramen; S Tazuma; T Ohya; G Kajiyama
Journal:  J Gastroenterol       Date:  1995-08       Impact factor: 7.527

3.  Bile lipid secretion in isolated perfused rat liver. A model for metabolic studies.

Authors:  J C Henry; N Domingo; F Chanussot; H Lafont; J C Hauton; J P Cano
Journal:  Lipids       Date:  1987-01       Impact factor: 1.880
  3 in total

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