Literature DB >> 3937520

Gene expression of ornithine decarboxylase in L1210 leukaemia cells exposed to DL-2-difluoromethylornithine in the presence of cadaverine.

L Alhonen-Hongisto, R Sinervirta, O A Jänne, J Jänne.   

Abstract

Cultured mouse L1210 leukaemia cells treated with DL-2-difluoromethylornithine, an irreversible inhibitor of ornithine decarboxylase (EC 4.1.1.17), in the presence of micromolar concentrations of cadaverine, started to overproduce ornithine decarboxylase after an exposure of several weeks. The more than 60-fold excess of the enzyme protein in the drug-treated cells apparently resulted from a strikingly enhanced accumulation of mRNA for the enzyme associated with only a modest (about 2-fold) gene amplification.

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Year:  1985        PMID： 3937520      PMCID： PMC1152922          DOI： 10.1042/bj2320605

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  18 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  A general method for isolation of high molecular weight DNA from eukaryotes.

Authors:  N Blin; D W Stafford
Journal:  Nucleic Acids Res       Date:  1976-09       Impact factor: 16.971

3.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

Review 4.  Use of the dansyl reaction in biochemical analysis.

Authors:  N Seiler
Journal:  Methods Biochem Anal       Date:  1970

5.  Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.

Authors:  P S Thomas
Journal:  Proc Natl Acad Sci U S A       Date:  1980-09       Impact factor: 11.205

6.  Replacement of natural polyamines by cadaverine and its aminopropyl derivatives in Ehrlich ascites carcinoma cells.

Authors:  L Alhonen-Hongisto; P Seppänen; E Hölttä; J Jänne
Journal:  Biochem Biophys Res Commun       Date:  1982-05-31       Impact factor: 3.575

7.  Ornithine decarboxylase in mouse kidney. Purification, characterization, and radioimmunological determination of the enzyme protein.

Authors:  V V Isomaa; A E Pajunen; C W Bardin; O A Jänne
Journal:  J Biol Chem       Date:  1983-06-10       Impact factor: 5.157

8.  Suppression of the formation of polyamines and macromolecules by DL-alpha-difluoromethylornithine and methylglyoxal bis(guanylhydrazone) in phytohaemagglutinin-activated human lymphocytes.

Authors:  E Hölttä; J Jänne; T Hovi
Journal:  Biochem J       Date:  1979-01-15       Impact factor: 3.857

9.  Ornithine decarboxylase mRNA in mouse kidney: a low abundancy gene product regulated by androgens with rapid kinetics.

Authors:  O A Jänne; K K Kontula; V V Isomaa; C W Bardin
Journal:  Ann N Y Acad Sci       Date:  1984       Impact factor: 5.691

10.  Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA.

Authors:  C Auffray; F Rougeon
Journal:  Eur J Biochem       Date:  1980-06
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  6 in total

1.  Mouse and human ornithine decarboxylase genes. Methylation polymorphism and amplification.

Authors:  L Alhonen-Hongisto; P Leinonen; R Sinervirta; R Laine; R Winqvist; K Alitalo; O A Jänne; J Jänne
Journal:  Biochem J       Date:  1987-02-15       Impact factor: 3.857

2.  Multiple mechanisms are responsible for altered expression of ornithine decarboxylase in overproducing variant cells.

Authors:  L McConlogue; S L Dana; P Coffino
Journal:  Mol Cell Biol       Date:  1986-08       Impact factor: 4.272

3.  Human myeloma cells acquire resistance to difluoromethylornithine by amplification of ornithine decarboxylase gene.

Authors:  P Leinonen; L Alhonen-Hongisto; R Laine; O A Jänne; J Jänne
Journal:  Biochem J       Date:  1987-02-15       Impact factor: 3.857

4.  Levels of ornithine decarboxylase genomic sequences, heterogeneous nuclear RNA and mRNA in human myeloma cells resistant to alpha-difluoromethylornithine.

Authors:  I M Hyttinen; M Halmekytö; L Alhonen; J Jänne
Journal:  Biochem J       Date:  1991-09-15       Impact factor: 3.857

5.  Characterization of difluoromethylornithine-resistant mouse and human tumour cell lines.

Authors:  A Hirvonen; T Eloranta; T Hyvönen; L Alhonen; J Jänne
Journal:  Biochem J       Date:  1989-03-15       Impact factor: 3.857

6.  Cadaverine supplementation during a chronic exposure to difluoromethylornithine allows an overexpression, but prevents gene amplification, of ornithine decarboxylase in L1210 mouse leukaemia cells.

Authors:  L Alhonen-Hongisto; A Hirvonen; R Sinervirta; J Jänne
Journal:  Biochem J       Date:  1987-11-01       Impact factor: 3.857
  6 in total

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