Literature DB >> 3933923

Localization and expression of transformed DNA sequences within heat shock puffs of Drosophila melanogaster.

J A Simon, C A Sutton, J T Lis.   

Abstract

In situ hybridization at high resolution with biotin-labeled DNA was used to locate specific transcriptional units within the chromosomal puffs of normal heat shock loci and of new heat shock loci generated by transformation. This method resolves copies of the hsp70 gene that are separated by 40 kb within the 87C heat shock locus. In the case of two new puff loci generated by transformation, the heat-activated transcript is encoded by sequences that reside entirely within the puff domain and the activated promoter is positioned asymmetrically within the puff. However, an adjacent promoter and its transcriptional unit which do not appear to be induced by heat shock are also within the puff. These results support the idea that a chromosomal puff is a structure that facilitates or results from vigorous transcription, but that the structural change alone is insufficient to induce transcription.

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Year:  1985        PMID： 3933923     DOI： 10.1007/bf01259442

Source DB:  PubMed          Journal:  Chromosoma        ISSN： 0009-5915            Impact factor:   4.316


  22 in total

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Journal:  Chromosoma       Date:  1964-04-01       Impact factor: 4.316

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4.  Genetic transformation of Drosophila with transposable element vectors.

Authors:  G M Rubin; A C Spradling
Journal:  Science       Date:  1982-10-22       Impact factor: 47.728

5.  Upstream elements necessary for optimal function of the hsp 70 promoter in transformed flies.

Authors:  R Dudler; A A Travers
Journal:  Cell       Date:  1984-09       Impact factor: 41.582

6.  The use of promoter fusions in Drosophila genetics: isolation of mutations affecting the heat shock response.

Authors:  J J Bonner; C Parks; J Parker-Thornburg; M A Mortin; H R Pelham
Journal:  Cell       Date:  1984-07       Impact factor: 41.582

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Authors:  C S Parker; J Topol
Journal:  Cell       Date:  1984-05       Impact factor: 41.582

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Authors:  J T Lis; L Prestidge; D S Hogness
Journal:  Cell       Date:  1978-08       Impact factor: 41.582

9.  Two closely linked transcription units within the 63B heat shock puff locus of D. melanogaster display strikingly different regulation.

Authors:  D O'Connor; J T Lis
Journal:  Nucleic Acids Res       Date:  1981-10-10       Impact factor: 16.971

10.  New heat shock puffs and beta-galactosidase activity resulting from transformation of Drosophila with an hsp70-lacZ hybrid gene.

Authors:  J T Lis; J A Simon; C A Sutton
Journal:  Cell       Date:  1983-12       Impact factor: 41.582
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  9 in total

1.  Studies of the role of the Drosophila scs and scs' insulators in defining boundaries of a chromosome puff.

Authors:  Emily J Kuhn; Craig M Hart; Pamela K Geyer
Journal:  Mol Cell Biol       Date:  2004-02       Impact factor: 4.272

2.  Chromosome structure in four wild-type polytene tissues of Drosophila melanogaster. The 87A and 87C heat shock loci are induced unequally in the midgut in a manner dependent on growth temperature.

Authors:  M Hochstrasser
Journal:  Chromosoma       Date:  1987       Impact factor: 4.316

3.  Structure and expression of ubiquitin genes of Drosophila melanogaster.

Authors:  H S Lee; J A Simon; J T Lis
Journal:  Mol Cell Biol       Date:  1988-11       Impact factor: 4.272

4.  Potential role for a FTZ-F1 steroid receptor superfamily member in the control of Drosophila metamorphosis.

Authors:  G Lavorgna; F D Karim; C S Thummel; C Wu
Journal:  Proc Natl Acad Sci U S A       Date:  1993-04-01       Impact factor: 11.205

5.  High-resolution localization of Drosophila Spt5 and Spt6 at heat shock genes in vivo: roles in promoter proximal pausing and transcription elongation.

Authors:  E D Andrulis; E Guzmán; P Döring; J Werner; J T Lis
Journal:  Genes Dev       Date:  2000-10-15       Impact factor: 11.361

6.  Transcription factor and polymerase recruitment, modification, and movement on dhsp70 in vivo in the minutes following heat shock.

Authors:  Amber K Boehm; Abbie Saunders; Janis Werner; John T Lis
Journal:  Mol Cell Biol       Date:  2003-11       Impact factor: 4.272

7.  Ultrastructural analysis of polytene chromatin of Drosophila melanogaster reveals clusters of tightly linked co-expressed genes.

Authors:  E J Hager; O L Miller
Journal:  Chromosoma       Date:  1991-03       Impact factor: 4.316

8.  Localization of specific repetitive DNA sequences in individual rice chromosomes.

Authors:  H K Wu; M C Chung; T Y Wu; C N Ning; R Wu
Journal:  Chromosoma       Date:  1991-06       Impact factor: 4.316

9.  Protein composition of interband regions in polytene and cell line chromosomes of Drosophila melanogaster.

Authors:  Sergey A Demakov; Tatyana Yu Vatolina; Vladimir N Babenko; Valery F Semeshin; Elena S Belyaeva; Igor F Zhimulev
Journal:  BMC Genomics       Date:  2011-11-18       Impact factor: 3.969
  9 in total

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