Chromosome structure in four wild-type polytene tissues of Drosophila melanogaster. The 87A and 87C heat shock loci are induced unequally in the midgut in a manner dependent on growth temperature.

A systematic screen of wild-type Drosophila melanogaster larval organs has revealed three tissues besides the salivary gland with suitable polyteny for detailed cytogenetic analysis: the prothoracic gland, hindgut, and middle midgut. Chromosome banding patterns are very similar between tissues, but puffing patterns show considerable differences. In intact nuclei, oblique substructural elements can sometimes be detected in bands from some of the tissues. As a way of exploiting these newly characterized chromosomes, the heat shock puff response in midgut cells has been studied in detail. The puffing pattern is very similar to that in salivary glands, but an unexpected difference is found in the relative activity of the 87A7 and 87C1 loci, which contain the hsp70 genes. When larvae are raised at 16 degrees C, heat shocks ranging from 10 to 60 min induce only a weak midgut puff at 87A7 that is much smaller than that at 87C1, in contrast to other tissues where both are strongly induced. In pulse-labeled nuclei, an approximately five fold difference in transcriptional activity at the two loci is observed. However, when larvae are raised at 25 degrees C, the converse is found: the 87A7 puff is large, and little or no puffing is detectable at 87C1. Thus, in the midgut, heat shock induced puffing at these two loci is inversely modulated by a mechanism dependent on growth temperature.