Literature DB >> 3928535

Detection and separation of the submetacentric marker chromosome of the WALKER (W-256) carcinoma using flow cytometry and sorting.

K J Hutter, M Stoehr.   

Abstract

The chromosomes of WALKER (W-256) carcinoma cells have been separated into different DNA subclasses using DAPI for quantitative DNA staining and laser flow cytometry. The submetacentric marker chromosome could be isolated and its DNA content was determined to be 1.3 pg. One microgram marker DNA was obtained after separation of about 750 000 marker chromosomes by means of electronic flow sorting. The chromosomal composition of sorted fractions was analyzed by microscopy following banding of sorted chromosomes. The average morphological purity obtained was about 83%.

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Year:  1985        PMID： 3928535     DOI： 10.1007/bf02450482

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  13 in total

1.  Chromosome measurement and sorting by flow systems.

Authors:  J W Gray; A V Carrano; L L Steinmetz; M A Van Dilla; D H Moore; B H Mayall; M L Mendelsohn
Journal:  Proc Natl Acad Sci U S A       Date:  1975-04       Impact factor: 11.205

2.  Extended application of flow microfluorometry by means of dual laser excitation.

Authors:  M Stöhr; H Eipel; K Goerttler; M Vogt-Schaden
Journal:  Histochemistry       Date:  1977-04-04

3.  Interaction of the RNA polymerase inhibitor chromomycin with DNA.

Authors:  W Behr; K Honikel; G Hartmann
Journal:  Eur J Biochem       Date:  1969-05-01

4.  DAPI fluorescence of plant chromosomes prestained with actinomycin D.

Authors:  D Schweizer
Journal:  Exp Cell Res       Date:  1976-10-15       Impact factor: 3.905

5.  A reliable preparation of mon-dispersed chromosome suspensions for flow cytometry.

Authors:  M Stoehr; K J Hutter; M Frank; K Goerttler
Journal:  Histochemistry       Date:  1982

6.  Separation of large quantities of chromosomes by velocity sedimentation at unit gravity.

Authors:  J G Collard; A Tulp; J H Hollander; F W Bauer; J Boezeman
Journal:  Exp Cell Res       Date:  1980-03       Impact factor: 3.905

7.  Homogeneously staining regions and double minutes in a human cell line: chromatin organization and DNA content.

Authors:  G Bahr; F Gilbert; G Balaban; W Engler
Journal:  J Natl Cancer Inst       Date:  1983-10       Impact factor: 13.506

8.  Phenotypic and genotypic characteristics of a cell line from a squamous cell carcinoma of human skin.

Authors:  P Boukamp; W Tilgen; R T Dzarlieva; D Breitkreutz; D Haag; R K Riehl; A Bohnert; N E Fusenig
Journal:  J Natl Cancer Inst       Date:  1982-03       Impact factor: 13.506

9.  The Heidelberg flow analyzer and sorter (HEIFAS) approach on the prescreening of uterine cancer.

Authors:  M Stöhr; K Goerttler
Journal:  J Histochem Cytochem       Date:  1979-01       Impact factor: 2.479

10.  Assignment of snRNA gene sequences to the large chromosomes of rat kangaroo and chinese hamster isolated by flow cytometric sorting.

Authors:  N Blin; M Stöhr; K J Hutter; A Alonso; K Goerttler
Journal:  Chromosoma       Date:  1982       Impact factor: 4.316
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  5 in total

1.  Specific and rapid enumeration of viable but nonculturable and viable-culturable gram-negative bacteria by using flow cytometry.

Authors:  Mohiuddin M Taimur Khan; Barry H Pyle; Anne K Camper
Journal:  Appl Environ Microbiol       Date:  2010-06-11       Impact factor: 4.792

2.  Karyotypic change from heteroploidy to near diploidy associated with development of cisplatin resistance in a rat ovarian tumour cell line.

Authors:  G Chen; K J Hutter; J Bullerdiek; W J Zeller
Journal:  J Cancer Res Clin Oncol       Date:  1991       Impact factor: 4.553

3.  Detection and chromosomal assignment of SV40-DNA integration in Chinese hamster cell lines by chromosome sorting and dot blot hybridization.

Authors:  K J Hutter; H Klefenz; K Goerttler
Journal:  Histochemistry       Date:  1990

4.  On-line sorting of human chromosomes by centromeric index, and identification of sorted populations by GTG-banding and fluorescent in situ hybridization.

Authors:  G A Boschman; W Rens; E Manders; C van Oven; G W Barendsen; J A Aten
Journal:  Hum Genet       Date:  1990-06       Impact factor: 4.132

5.  A comparison of the effect of 5-bromodeoxyuridine substitution on 33258 Hoechst- and DAPI-fluorescence of isolated chromosomes by bivariate flow karyotyping.

Authors:  C H Buys; J Mesa; A Y van der Veen; J A Aten
Journal:  Histochemistry       Date:  1986
  5 in total

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