Literature DB >> 3928445

Nucleotide sequence of the dihydrofolate reductase gene of methotrexate-resistant Lactobacillus casei.

J Andrews, G M Clore, R W Davies, A M Gronenborn, B Gronenborn, D Kalderon, P C Papadopoulos, S Schäfer, P F Sims, R Stancombe.   

Abstract

The nucleotide sequence of the dihydrofolate reductase (DHFR) gene of a methotrexate-resistant strain of Lactobacillus casei, which is the source of DHFR for nuclear magnetic resonance (NMR) studies, has been determined. The derived amino acid sequence differs from that obtained by protein sequencing by the presence of aspartic acid instead of asparagine at position 8 and proline instead of leucine at position 90. The nucleotide sequences of 320-bp 5' and 335-bp 3' flanking regions of this gene have also been determined.

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Year:  1985        PMID： 3928445     DOI： 10.1016/0378-1119(85)90174-x

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  9 in total

1.  Structure and dynamics in solution of the complex of Lactobacillus casei dihydrofolate reductase with the new lipophilic antifolate drug trimetrexate.

Authors:  V I Polshakov; B Birdsall; T A Frenkiel; A R Gargaro; J Feeney
Journal:  Protein Sci       Date:  1999-03       Impact factor: 6.725

2.  Calmodulin tagging provides a general method of using lanthanide induced magnetic field orientation to observe residual dipolar couplings in proteins in solution.

Authors:  J Feeny; B Birdsall; A F Bradbury; R R Biekofsky; P M Bayley
Journal:  J Biomol NMR       Date:  2001-09       Impact factor: 2.835

3.  Biosynthesis of D-alanyl-lipoteichoic acid: cloning, nucleotide sequence, and expression of the Lactobacillus casei gene for the D-alanine-activating enzyme.

Authors:  M P Heaton; F C Neuhaus
Journal:  J Bacteriol       Date:  1992-07       Impact factor: 3.490

4.  3D 13C/1H NMR-based assignments for side-chain resonances of Lactobacillus casei dihydrofolate reductase. Evidence for similarities between the solution and crystal structures of the enzyme.

Authors:  A Soteriou; M D Carr; T A Frenkiel; J E McCormick; C J Bauer; D Sali; B Birdsall; J Feeney
Journal:  J Biomol NMR       Date:  1993-09       Impact factor: 2.835

5.  Cloning and molecular analysis of the dihydrofolate reductase gene from Lactococcus lactis.

Authors:  K Leszczyńska; A Bolhuis; K Leenhouts; G Venema; P Cegłowski
Journal:  Appl Environ Microbiol       Date:  1995-02       Impact factor: 4.792

6.  Expression and nucleotide sequence of the Lactobacillus bulgaricus beta-galactosidase gene cloned in Escherichia coli.

Authors:  B F Schmidt; R M Adams; C Requadt; S Power; S E Mainzer
Journal:  J Bacteriol       Date:  1989-02       Impact factor: 3.490

7.  3H-n.m.r. studies of multiple conformations and dynamic processes in complexes of folate and methotrexate with Lactobacillus casei dihydrofolate reductase.

Authors:  N Curtis; S Moore; B Birdsall; J Bloxsidge; C L Gibson; J R Jones; J Feeney
Journal:  Biochem J       Date:  1994-10-15       Impact factor: 3.857

8.  Isolation and expression of the Pneumocystis carinii dihydrofolate reductase gene.

Authors:  J C Edman; U Edman; M Cao; B Lundgren; J A Kovacs; D V Santi
Journal:  Proc Natl Acad Sci U S A       Date:  1989-11       Impact factor: 11.205

9.  NMR structures of apo L. casei dihydrofolate reductase and its complexes with trimethoprim and NADPH: contributions to positive cooperative binding from ligand-induced refolding, conformational changes, and interligand hydrophobic interactions.

Authors:  James Feeney; Berry Birdsall; Nadezhda V Kovalevskaya; Yegor D Smurnyy; Emna M Navarro Peran; Vladimir I Polshakov
Journal:  Biochemistry       Date:  2011-04-14       Impact factor: 3.162
  9 in total

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