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Literature DB >> 3923333

The SOS Chromotest, a colorimetric bacterial assay for genotoxins: procedures.

Abstract

The SOS Chromotest is a quantitative bacterial colorimetric assay for genotoxins. Substantial validation is now available (Quillardet et al., 1985). We describe here in detail the tester strain as well as the effects of the variation of some parameters on the assay. We report a simple spot-test procedure as well as a new standard procedure which incorporate recent technical improvements aimed at simplifying the assay further.

Entities: Disease

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Year: 1985 PMID： 3923333 DOI： 10.1016/0165-1161(85)90020-2

Source DB: PubMed Journal: Mutat Res ISSN： 0027-5107 Impact factor: 2.433

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Cited

52 in total

1. The role of p-aminobenzoic acid in DNA protection against oxidants.

Authors: S V Vasil'eva; E V Makhova; E Y Moshkovskaya; G P Zhizhina
Journal: Dokl Biol Sci Date: 2000 Nov-Dec

2. Does UVB radiation induce SoxS gene expression in Escherichia coli cells?

Authors: A A Gomes; L M B O Asad; I Felzenszwalb; A C Leitão; A B Silva; H C R Guillobel; N R Asad
Journal: Radiat Environ Biophys Date: 2004-09-14 Impact factor: 1.925

3. Immunological quantification of RecA protein in cell extracts of E. coli after exposure to chemical mutagens or UV radiation.

Authors: I Fridrichová; A Kovarík; O Rosskopfová
Journal: Folia Microbiol (Praha) Date: 1992 Impact factor: 2.099

4. Construction of a ColD cda promoter-based SOS-green fluorescent protein whole-cell biosensor with higher sensitivity toward genotoxic compounds than constructs based on recA, umuDC, or sulA promoters.

Authors: Anders Norman; Lars Hestbjerg Hansen; Søren J Sørensen
Journal: Appl Environ Microbiol Date: 2005-05 Impact factor: 4.792

5. Iron-mediated induction of the SOS responses by hydrogen peroxide.

Authors: R Q Zhou; X A Sun; D T Tang
Journal: Bull Environ Contam Toxicol Date: 1991-04 Impact factor: 2.151

6. Nitro musk compounds genotoxic activity : Genotoxicity testing of nitro musks with the SOS-chromotest and the sister-chromatid exchange test.

Authors: S Kevekordes; K Grahl; A Zaulig; H Dunkelberg
Journal: Environ Sci Pollut Res Int Date: 1996-12 Impact factor: 4.223

The SOS Chromotest, a colorimetric bacterial assay for genotoxins: procedures.

1. The role of p-aminobenzoic acid in DNA protection against oxidants.

2. Does UVB radiation induce SoxS gene expression in Escherichia coli cells?

3. Immunological quantification of RecA protein in cell extracts of E. coli after exposure to chemical mutagens or UV radiation.

4. Construction of a ColD cda promoter-based SOS-green fluorescent protein whole-cell biosensor with higher sensitivity toward genotoxic compounds than constructs based on recA, umuDC, or sulA promoters.

5. Iron-mediated induction of the SOS responses by hydrogen peroxide.

6. Nitro musk compounds genotoxic activity : Genotoxicity testing of nitro musks with the SOS-chromotest and the sister-chromatid exchange test.

7. Ecotoxicity and genotoxicity assessment of exhaust particulates from diesel-powered buses.

8. Expression of the dnaN and dnaQ genes of Escherichia coli is inducible by mitomycin C.

9. Genotoxic and Cytotoxic Safety Evaluation of Papain (Carica papaya L.) Using In Vitro Assays.

10. Expression of the dnaA gene of Escherichia coli is inducible by DNA damage.