Literature DB >> 3918225

Characterization of extracellular phospholipase A2 in rheumatoid synovial fluid.

P Vadas, E Stefanski, W Pruzanski.   

Abstract

Phospholipase A2 (PLA2) activity has now been identified in rheumatoid synovial fluids. This PLA2 is a calcium-requiring protein of MW 11,000 with a neutral pH optimum. Its activity was inhibited by high concentrations of Mg2+, and by the active site-directed histidine reagent p-bromophenacyl bromide. Ionic and nonionic detergents, or the sulfhydryl reagent dithiothreitol caused loss of enzyme activity. Synovial fluid PLA2 did not interact with sulphated mucopolysaccharides such as heparin or chondroitin sulphate. Release and sequestration of PLA2 in the joint space may contribute to the characteristic rheumatoid inflammatory changes.

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Year:  1985        PMID： 3918225     DOI： 10.1016/0024-3205(85)90640-x

Source DB:  PubMed          Journal:  Life Sci        ISSN： 0024-3205            Impact factor:   5.037


  27 in total

1.  The molecular basis of phosphatidylcholine preference of human group-V phospholipase A2.

Authors:  K P Kim; S K Han; M Hong; W Cho
Journal:  Biochem J       Date:  2000-06-15       Impact factor: 3.857

2.  Enzymatic activity and immunoreactivity of extracellular phospholipase A2 in inflammatory synovial fluids.

Authors:  W Pruzanski; K Scott; G Smith; I Rajkovic; E Stefanski; P Vadas
Journal:  Inflammation       Date:  1992-10       Impact factor: 4.092

Review 3.  Synovial fluid phospholipase A2s and inflammation.

Authors:  H Gonzalez-Buritica; M A Khamashita; G R Hughes
Journal:  Ann Rheum Dis       Date:  1989-04       Impact factor: 19.103

4.  Comparison of group I and II soluble phospholipases A2 activities on phagocytic functions of human polymorphonuclear and mononuclear phagocytes.

Authors:  W Pruzanski; S Saito; E Stefanski; P Vadas
Journal:  Inflammation       Date:  1991-04       Impact factor: 4.092

5.  CoA-independent transacylase has characteristics distinct from those of PLA2 enzymes.

Authors:  J D Winkler; C M Sung; L Huang
Journal:  Agents Actions       Date:  1991-09

6.  Proinflammatory secreted phospholipase A2 type IIA (sPLA-IIA) induces integrin activation through direct binding to a newly identified binding site (site 2) in integrins αvβ3, α4β1, and α5β1.

Authors:  Masaaki Fujita; Kan Zhu; Chitose K Fujita; Min Zhao; Kit S Lam; Mark J Kurth; Yoko K Takada; Yoshikazu Takada
Journal:  J Biol Chem       Date:  2014-11-14       Impact factor: 5.157

7.  Phospholipase activity in intact rat glycogen-elicited neutrophils.

Authors:  J Chang; E Blazek; A J Lewis
Journal:  Agents Actions       Date:  1986-01

8.  The proinflammatory effect of intra-articular injection of soluble human and venom phospholipase A2.

Authors:  P Vadas; W Pruzanski; J Kim; V Fornasier
Journal:  Am J Pathol       Date:  1989-04       Impact factor: 4.307

9.  Extracellular phospholipase A2: causative agent in circulatory collapse of septic shock?

Authors:  P Vadas; W Pruzanski; E Stefanski
Journal:  Agents Actions       Date:  1988-07

10.  Circulating phospholipase A2 activity associated with sepsis and septic shock is indistinguishable from that associated with rheumatoid arthritis.

Authors:  J A Green; G M Smith; R Buchta; R Lee; K Y Ho; I A Rajkovic; K F Scott
Journal:  Inflammation       Date:  1991-10       Impact factor: 4.092
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