Literature DB >> 3918007

Isolation and properties of beta-glucosidase from Ruminococcus albus.

K Ohmiya, M Shirai, Y Kurachi, S Shimizu.   

Abstract

An enzyme active against p-nitrophenyl-beta-D-glucoside was purified from logarithmic-phase cells of Ruminococcus albus cultivated in a medium containing ball-milled cellulose. The purification yielded homogeneous enzyme after an approximately 520-fold increase in specific activity and a 9% yield. The enzyme was identified as a beta-glucosidase because it can hydrolyze cellobiose and cellooligosaccharides to glucose from the nonreducing ends.

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Year:  1985        PMID: 3918007      PMCID: PMC214890          DOI: 10.1128/jb.161.1.432-434.1985

Source DB:  PubMed          Journal:  J Bacteriol        ISSN: 0021-9193            Impact factor:   3.490


  9 in total

1.  Comparison of Extracellular Cellulase Activities of Clostridium thermocellum LQRI and Trichoderma reesei QM9414.

Authors:  T K Ng; J G Zeikus
Journal:  Appl Environ Microbiol       Date:  1981-08       Impact factor: 4.792

2.  Enzymatic studies of pure cultures of rumen microorganisms.

Authors:  A E Joyner; R L Baldwin
Journal:  J Bacteriol       Date:  1966-11       Impact factor: 3.490

3.  Cellulose degradation by Ruminococcus.

Authors:  J M Leatherwood
Journal:  Fed Proc       Date:  1973-07

4.  The mechanism of enzymatic cellulose degradation. Isolation and some properties of a beta-glucosidase from Trichoderma viride.

Authors:  L E Berghem; L G Pettersson
Journal:  Eur J Biochem       Date:  1974-07-15

5.  Purification and properties of cellobiosidase from Ruminococcus albus.

Authors:  K Ohmiya; M Shimizu; M Taya; S Shimizu
Journal:  J Bacteriol       Date:  1982-04       Impact factor: 3.490

6.  Rumen bacterial degradation of forage cell walls investigated by electron microscopy.

Authors:  D E Akin; H E Amos
Journal:  Appl Microbiol       Date:  1975-05

7.  Cellulolytic activity of the rumen bacterium Bacteroides succinogenes.

Authors:  D Groleau; C W Forsberg
Journal:  Can J Microbiol       Date:  1981-05       Impact factor: 2.419

8.  Purification and characterization of an endoglucanase (1,4-beta-D-glucan glucanohydrolase) from Clostridium thermocellum.

Authors:  T K Ng; J G Zeikus
Journal:  Biochem J       Date:  1981-11-01       Impact factor: 3.857

9.  Hydrophobic interaction chromatography on uncharged Sepharose derivatives. Effects of neutral salts on the adsorption of proteins.

Authors:  J Rosengren; S Hjertén
Journal:  J Chromatogr       Date:  1977-01-21
  9 in total
  20 in total

1.  Physical mapping of transposon Tn5 insertions defines a gene cluster functional in nitrous oxide respiration by Pseudomonas stutzeri.

Authors:  A Viebrock; W G Zumft
Journal:  J Bacteriol       Date:  1987-10       Impact factor: 3.490

2.  Cellodextrin utilization and beta-glucosidase production by Bacteroides polypragmatus.

Authors:  C R MacKenzie; G B Patel
Journal:  Arch Microbiol       Date:  1986-06       Impact factor: 2.552

Review 3.  Anaerobic digestion of lignocellulosic biomass and wastes. Cellulases and related enzymes.

Authors:  W S Adney; C J Rivard; S A Ming; M E Himmel
Journal:  Appl Biochem Biotechnol       Date:  1991-08       Impact factor: 2.926

4.  Intergeneric Protoplast Fusion between Ruminococcus albus and an Anaerobic Recombinant, FE7.

Authors:  W Chen; K Nagashima; T Kajino; K Ohmiya; S Shimizu
Journal:  Appl Environ Microbiol       Date:  1988-05       Impact factor: 4.792

5.  Purification and Characterization of an Aspecific Glycoside Hydrolase from the Anaerobic Ruminal Fungus Neocallimastix frontalis.

Authors:  M Hebraud; M Fevre
Journal:  Appl Environ Microbiol       Date:  1990-10       Impact factor: 4.792

6.  Isolation of a Cellodextrinase from Bacteroides succinogenes.

Authors:  L Huang; C W Forsberg
Journal:  Appl Environ Microbiol       Date:  1987-05       Impact factor: 4.792

7.  Molecular Cloning and Expression of Cellulase Genes from Ruminococcus albus 8 in Escherichia coli Bacteriophage lambda.

Authors:  G T Howard; B A White
Journal:  Appl Environ Microbiol       Date:  1988-07       Impact factor: 4.792

8.  Cellulolytic Activity of Clostridium acetobutylicum.

Authors:  S F Lee; C W Forsberg; L N Gibbins
Journal:  Appl Environ Microbiol       Date:  1985-08       Impact factor: 4.792

9.  Metabolic mechanism of mannan in a ruminal bacterium, Ruminococcus albus, involving two mannoside phosphorylases and cellobiose 2-epimerase: discovery of a new carbohydrate phosphorylase, β-1,4-mannooligosaccharide phosphorylase.

Authors:  Ryosuke Kawahara; Wataru Saburi; Rei Odaka; Hidenori Taguchi; Shigeaki Ito; Haruhide Mori; Hirokazu Matsui
Journal:  J Biol Chem       Date:  2012-10-23       Impact factor: 5.157

10.  Properties of a recombinant beta-glucosidase from polycentric anaerobic fungus Orpinomyces PC-2 and its application for cellulose hydrolysis.

Authors:  Xin-Liang Li; Lars G Ljungdahl; Eduardo A Ximenes; Huizhong Chen; Carlos R Felix; Michael A Cotta; Bruce S Dien
Journal:  Appl Biochem Biotechnol       Date:  2004       Impact factor: 2.926

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