| Literature DB >> 3916735 |
P Cottrelle1, M Cool, P Thuriaux, V L Price, D Thiele, J M Buhler, P Fromageot.
Abstract
Two genes, TEF1 and TEF2, encode the protein elongation factor EF-1 alpha in the yeast Saccharomyces cerevisiae. We have generated yeast haploid strains containing either TEF1 or TEF2 interrupted by insertion of a large piece of foreign DNA. Cells which contain either one functional copy of the EF-1 alpha genes are viable. In contrast, attempts to isolate a yeast haploid strain with both TEF1 and TEF2 inactivated have failed suggesting that the double gene disruption is a lethal event.Entities:
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Year: 1985 PMID: 3916735 DOI: 10.1007/bf00449823
Source DB: PubMed Journal: Curr Genet ISSN: 0172-8083 Impact factor: 3.886