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Literature DB >> 3916718

Rapid alkaline preparation for yeast circular covalently closed DNA molecules.

Abstract

The alkaline preparation of prokaryotic plasmids (Birnboim and Doly, 1979) has been here adapted to yeast. By simple denaturation and renaturation steps we recovered, from Saccharomyces cerevisiae and Schizosaccharomyces pombe, a population of nucleic acid molecules highly enriched in circular forms. In S. cerevisiae killer strains it is possible to copurify double stranded RNA molecules. The overall recovery was estimated to be 10-30% of the total circular molecules.

Entities: Species

Mesh：

Substances：
DNA, Circular
DNA, Fungal

Year: 1985 PMID： 3916718 DOI： 10.1007/bf00436959

Source DB: PubMed Journal: Curr Genet ISSN： 0172-8083 Impact factor: 3.886

13 in total

1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

2. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Authors: H C Birnboim; J Doly
Journal: Nucleic Acids Res Date: 1979-11-24 Impact factor: 16.971

3. Characterization of a new class of circular DNA molecules in yeast.

Authors: M Guerineau; C Grandchamp; C Paoletti; P Slonimski
Journal: Biochem Biophys Res Commun Date: 1971-02-05 Impact factor: 3.575

4. H5 Histone and DNA-relaxing enzyme of chicken erythrocytes. Interaction with superhelical DNA.

Authors: M Bina-Stein; T Vogel; D S Singer; M F Singer
Journal: J Biol Chem Date: 1976-12-10 Impact factor: 5.157

5. Nucleotide sequence of the yeast plasmid.

Authors: J L Hartley; J E Donelson
Journal: Nature Date: 1980-08-28 Impact factor: 49.962

6. Analysis of chromosomal integration and deletions of yeast plasmids.

Authors: J R Cameron; P Philippsen; R W Davis
Journal: Nucleic Acids Res Date: 1977 Impact factor: 16.971

7. Hybridizable sequences between cytoplasmic ribosomal RNAs and 3 micron circular DNAs of Saccharomyces cerevisiae and Torulopsis glabrata.

Authors: G D Clark-Walker; A A Azad
Journal: Nucleic Acids Res Date: 1980-03-11 Impact factor: 16.971

Rapid alkaline preparation for yeast circular covalently closed DNA molecules.

1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

2. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

3. Characterization of a new class of circular DNA molecules in yeast.

4. H5 Histone and DNA-relaxing enzyme of chicken erythrocytes. Interaction with superhelical DNA.

5. Nucleotide sequence of the yeast plasmid.

6. Analysis of chromosomal integration and deletions of yeast plasmids.

7. Hybridizable sequences between cytoplasmic ribosomal RNAs and 3 micron circular DNAs of Saccharomyces cerevisiae and Torulopsis glabrata.

8. Non-Mendelian mutation allowing ureidosuccinic acid uptake in yeast.

9. [Induction of saccharomyces cerevisiae extrachromosomal-DNA].

10. Ty1 extrachromosomal circular copies in Saccharomyces cerevisiae.

1. Extrachromosomal elements cause a reduced division potential in nib 1 strains of Saccharomyces cerevisiae.

2. The smt-0 mutation which abolishes mating-type switching in fission yeast is a deletion.

3. The REV1 gene of Saccharomyces cerevisiae: isolation, sequence, and functional analysis.

4. Identification of autonomously replicating circular subtelomeric Y' elements in Saccharomyces cerevisiae.