Literature DB >> 3912516

A region flanking the GAL7 gene and a binding site for GAL4 protein as upstream activating sequences in yeast.

Y Lorch, R D Kornberg.   

Abstract

A region of DNA 116 to 271 base-pairs upstream from the GAL7 gene of Saccharomyces cerevisiae activates transcription from a heterologous promoter and does so in either orientation, showing that the GAL7 upstream region contains an upstream activating sequence (UAS). The level of transcription obtained with two GAL7 UAS's in tandem was only 1.3 times that with one. Previous studies of the GAL1-GAL10 intergenic region were indicative of two binding sites for the GAL4 positive regulatory protein; we find that a single (synthetic) site is capable of gene activation. The level of transcription obtained with the intact GAL1-GAL10 UAS was five times that with the single site.

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Year:  1985        PMID： 3912516     DOI： 10.1016/0022-2836(85)90400-0

Source DB:  PubMed          Journal:  J Mol Biol        ISSN： 0022-2836            Impact factor:   5.469


  19 in total

1.  Cis-acting elements sufficient for induction of FDH1 expression by formate in the methylotrophic yeast Candida boidinii.

Authors:  T Komeda; H Yurimoto; N Kato; Y Sakai; K Kondo
Journal:  Mol Genet Genomics       Date:  2003-10-08       Impact factor: 3.291

2.  Two systems of glucose repression of the GAL1 promoter in Saccharomyces cerevisiae.

Authors:  J S Flick; M Johnston
Journal:  Mol Cell Biol       Date:  1990-09       Impact factor: 4.272

3.  Upstream activation sequence-dependent alteration of chromatin structure and transcription activation of the yeast GAL1-GAL10 genes.

Authors:  M J Fedor; R D Kornberg
Journal:  Mol Cell Biol       Date:  1989-04       Impact factor: 4.272

4.  A yeast ARS-binding protein activates transcription synergistically in combination with other weak activating factors.

Authors:  A R Buchman; R D Kornberg
Journal:  Mol Cell Biol       Date:  1990-03       Impact factor: 4.272

5.  The yeast ROAM mutation--identification of the sequences mediating host gene activation and cell-type control in the yeast retrotransposon, Ty.

Authors:  P D Rathjen; A J Kingsman; S M Kingsman
Journal:  Nucleic Acids Res       Date:  1987-09-25       Impact factor: 16.971

6.  Connections between transcriptional activators, silencers, and telomeres as revealed by functional analysis of a yeast DNA-binding protein.

Authors:  A R Buchman; N F Lue; R D Kornberg
Journal:  Mol Cell Biol       Date:  1988-12       Impact factor: 4.272

7.  Interaction of GAL4 and GAL80 gene regulatory proteins in vitro.

Authors:  N F Lue; D I Chasman; A R Buchman; R D Kornberg
Journal:  Mol Cell Biol       Date:  1987-10       Impact factor: 4.272

Review 8.  A model fungal gene regulatory mechanism: the GAL genes of Saccharomyces cerevisiae.

Authors:  M Johnston
Journal:  Microbiol Rev       Date:  1987-12

9.  The positive and negative cis-acting elements for methanol regulation in the Pichia pastoris AOX2 gene.

Authors:  H Ohi; M Miura; R Hiramatsu; T Ohmura
Journal:  Mol Gen Genet       Date:  1994-06-03

10.  Mutations that inactivate a yeast transcriptional regulatory protein cluster in an evolutionarily conserved DNA binding domain.

Authors:  M Johnston; J Dover
Journal:  Proc Natl Acad Sci U S A       Date:  1987-04       Impact factor: 11.205
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