| Literature DB >> 3912508 |
E Carlemalm, W Villiger, J A Hobot, J D Acetarin, E Kellenberger.
Abstract
Lowicryl K4M and HM20 are methacrylate/acrylate based low temperature embedding resins for biological material which can be used in conjunction with either the progressive lowering of temperature (PLT) technique or with freeze-substitution. K4M and HM20 are applicable over a very extended temperature range, approximately 220 K to 340 K. With two new resins, K11M and HM23, one can reach even lower temperatures, c. 200 K. Freeze-substitution combined with low temperature embedding allows for very mild or no chemical fixation which seems to increase the sensitivity of immunocytochemical localization of antigens on sections.Entities:
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Year: 1985 PMID： 3912508 DOI： 10.1111/j.1365-2818.1985.tb02660.x
Source DB: PubMed Journal: J Microsc ISSN： 0022-2720 Impact factor: 1.758