Literature DB >> 3912370

Specific conjugation reactions of the oligosaccharide moieties of immunoglobulins.

D J O'Shannessy, R H Quarles.   

Abstract

Methods for the specific conjugation of both polyclonal antibodies and monoclonal antibodies via their carbohydrate moieties are described. Mild oxidation of the immunoglobulin with sodium periodate produces reactive aldehydes on the carbohydrate moieties. Subsequent reaction with hydrazide derivatives of biotin, fluorescent dyes, or enzymes produces stable antibody conjugates which retain full immunological activity. In addition, immunoaffinity supports can be prepared in the same manner using solid supports containing a hydrazide function.

Entities:  

Mesh：

Substances：

Year:  1985        PMID： 3912370

Source DB:  PubMed          Journal:  J Appl Biochem        ISSN： 0161-7354


  9 in total

Review 1.  Plasmon resonance methods in GPCR signaling and other membrane events.

Authors:  I D Alves; C K Park; V J Hruby
Journal:  Curr Protein Pept Sci       Date:  2005-08       Impact factor: 3.272

2.  Synthesis of site-specific methotrexate-IgG conjugates. Comparison of stability and antitumor activity with active-ester-based conjugates.

Authors:  J Kralovec; M Singh; M Mammen; A H Blair; T Ghose
Journal:  Cancer Immunol Immunother       Date:  1989       Impact factor: 6.968

3.  Pre-mRNA splicing by complementation with purified human U1, U2, U4/U6 and U5 snRNPs.

Authors:  A R Krainer
Journal:  Nucleic Acids Res       Date:  1988-10-25       Impact factor: 16.971

4.  Differential idiotype utilization for the in vivo type 14 capsular polysaccharide-specific Ig responses to intact Streptococcus pneumoniae versus a pneumococcal conjugate vaccine.

Authors:  Jesus Colino; Leah Duke; Swadhinya Arjunaraja; Quanyi Chen; Leyu Liu; Alexander H Lucas; Clifford M Snapper
Journal:  J Immunol       Date:  2012-06-15       Impact factor: 5.422

5.  Identification of major glycoconjugates from Mycobacterium bovis culture filtrate by biotin-hydrazide labeling.

Authors:  F Massó; E Varela; A Páez; E Zenteno; L F Montaño
Journal:  Glycoconj J       Date:  1998-09       Impact factor: 2.916

6.  Noncovalent association of protein and capsular polysaccharide on bacteria-sized latex beads as a model for polysaccharide-specific humoral immunity to intact gram-positive extracellular bacteria.

Authors:  Jesus Colino; Leah Duke; Clifford M Snapper
Journal:  J Immunol       Date:  2013-08-07       Impact factor: 5.422

7.  Oriented immobilization of periodate-oxidized monoclonal antibodies on amino and hydrazide derivatives of Eupergit C.

Authors:  G Fleminger; E Hadas; T Wolf; B Solomon
Journal:  Appl Biochem Biotechnol       Date:  1990-02       Impact factor: 2.926

8.  Oriented covalent immobilization of antibodies for measurement of intermolecular binding forces between zipper-like contact surfaces of split inteins.

Authors:  Mirco Sorci; Bareket Dassa; Hongwei Liu; Gaurav Anand; Amit K Dutta; Shmuel Pietrokovski; Marlene Belfort; Georges Belfort
Journal:  Anal Chem       Date:  2013-05-31       Impact factor: 6.986

9.  Regulation of Mel-CAM/MUC18 expression on melanocytes of different stages of tumor progression by normal keratinocytes.

Authors:  I M Shih; D E Elder; M Y Hsu; M Herlyn
Journal:  Am J Pathol       Date:  1994-10       Impact factor: 4.307
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.