Literature DB >> 10052587

Identification of major glycoconjugates from Mycobacterium bovis culture filtrate by biotin-hydrazide labeling.

F Massó1, E Varela, A Páez, E Zenteno, L F Montaño.   

Abstract

To identify Mycobacterium bovis glycoproteins, carbohydrates present in a delipidized M. bovis culture filtrate protein extract were biotin-hydrazide labeled. 11 carbohydrate-containing protein with a molecular weight of 15-, 19-, 25-, 32-, 35-, 39-, 42-, 48-, 52-, 58-, and 62-kDa were detected. The 52- and 32-kDa protein were deglycosylated by endoglycosidase-F.

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Year:  1998        PMID： 10052587     DOI： 10.1023/a:1006963116919

Source DB:  PubMed          Journal:  Glycoconj J        ISSN： 0282-0080            Impact factor:   2.916


  21 in total

Review 1.  Mycobacterial protein antigens: a compilation.

Authors:  D B Young; S H Kaufmann; P W Hermans; J E Thole
Journal:  Mol Microbiol       Date:  1992-01       Impact factor: 3.501

2.  Specificity of antibodies to immunodominant mycobacterial antigens in pulmonary tuberculosis.

Authors:  P S Jackett; G H Bothamley; H V Batra; A Mistry; D B Young; J Ivanyi
Journal:  J Clin Microbiol       Date:  1988-11       Impact factor: 5.948

3.  Selective labeling of functional groups on membrane proteins or glycoproteins using reactive biotin derivatives and 125I-streptavidin.

Authors:  E Roffman; L Meromsky; H Ben-Hur; E A Bayer; M Wilchek
Journal:  Biochem Biophys Res Commun       Date:  1986-04-14       Impact factor: 3.575

4.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

5.  The isolation by immunoabsorbent affinity chromatography and physicochemical characterization of Mycobacterium tuberculosis antigen 5.

Authors:  T M Daniel; P A Anderson
Journal:  Am Rev Respir Dis       Date:  1978-03

6.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

7.  Definition of the full extent of glycosylation of the 45-kilodalton glycoprotein of Mycobacterium tuberculosis.

Authors:  K M Dobos; K H Khoo; K M Swiderek; P J Brennan; J T Belisle
Journal:  J Bacteriol       Date:  1996-05       Impact factor: 3.490

8.  Detection of the low-density-lipoprotein receptor with biotin-low-density lipoprotein. A rapid new method for ligand blotting.

Authors:  D P Wade; B L Knight; A K Soutar
Journal:  Biochem J       Date:  1985-08-01       Impact factor: 3.857

9.  Purification and characterization of major antigens from a Mycobacterium bovis culture filtrate.

Authors:  T Fifis; C Costopoulos; A J Radford; A Bacic; P R Wood
Journal:  Infect Immun       Date:  1991-03       Impact factor: 3.441

10.  Evidence for glycosylation sites on the 45-kilodalton glycoprotein of Mycobacterium tuberculosis.

Authors:  K M Dobos; K Swiderek; K H Khoo; P J Brennan; J T Belisle
Journal:  Infect Immun       Date:  1995-08       Impact factor: 3.441
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