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Literature DB >> 3903213

Mutagenesis of bacteriophage T7 and T7 DNA by alkylation damage.

Abstract

We have developed a new assay for in vitro mutagenesis of bacteriophage T7 DNA that measures the generation of mutations in the specific T7 gene that codes for the phage ligase. This assay was used to examine mutagenesis caused by in vitro DNA synthesis in the presence of O6-methylguanosine triphosphate. Reversion of one of the newly generated ligase mutants by ethyl methanesulfonate was also tested.

Entities: Chemical Species

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Year: 1985 PMID： 3903213 PMCID： PMC252626

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

15 in total

Review 1. Bacteriophage T7.

Authors: F W Studier
Journal: Science Date: 1972-04-28 Impact factor: 47.728

2. Genetic studies of the lac repressor. IV. Mutagenic specificity in the lacI gene of Escherichia coli.

Authors: C Coulondre; J H Miller
Journal: J Mol Biol Date: 1977-12-15 Impact factor: 5.469

3. Genetic analysis of non-essential bacteriophage T7 genes.

Authors: F W Studier
Journal: J Mol Biol Date: 1973-09-15 Impact factor: 5.469

4. Possible relevance of O-6 alkylation of deoxyguanosine to the mutagenicity and carcinogenicity of nitrosamines and nitrosamides.

Authors: A Loveless
Journal: Nature Date: 1969-07-12 Impact factor: 49.962

5. In vivo mutagenesis by O6-methylguanine built into a unique site in a viral genome.

Authors: E L Loechler; C L Green; J M Essigmann
Journal: Proc Natl Acad Sci U S A Date: 1984-10 Impact factor: 11.205

6. In vitro packaging of bacteriophate T7 DNA synthesized in vitro.

Authors: W E Masker; N B Kuemmerle; D P Allison
Journal: J Virol Date: 1978-07 Impact factor: 5.103

7. Demethylation of O6-methylguanine in a synthetic DNA polymer by an inducible activity in Escherichia coli.

Authors: R S Foote; S Mitra; B C Pal
Journal: Biochem Biophys Res Commun Date: 1980-11-28 Impact factor: 3.575

8. A mutant of bacteriophage T7 deficient in polynucleotide ligase.

Authors: Y Masamune; G D Frenkel; C C Richardson
Journal: J Biol Chem Date: 1971-11-25 Impact factor: 5.157

9. In vitro packaging of UV radiation-damaged DNA from bacteriophage T7.

Authors: N B Kuemmerle; W E Masker
Journal: J Virol Date: 1977-09 Impact factor: 5.103

10. Repair of alkylated DNA in Escherichia coli. Methyl group transfer from O6-methylguanine to a protein cysteine residue.

Authors: M Olsson; T Lindahl
Journal: J Biol Chem Date: 1980-11-25 Impact factor: 5.157

3 in total