Literature DB >> 3902885

Comparison of polyclonal antiserum versus monoclonal antibodies for the rapid diagnosis of influenza A virus infections by immunofluorescence in clinical specimens.

I Shalit, P A McKee, H Beauchamp, J L Waner.   

Abstract

A pool of monoclonal antibodies was compared with polyclonal antiserum for the rapid detection of influenza A virus in 28 clinical specimens by immunofluorescence. Monoclonal antibodies showed higher sensitivity (69 versus 46%) and accuracy (86 versus 75%) and easier slide interpretation than did polyclonal antiserum. The procedure proved useful for rapid detection of a community outbreak of influenza A virus infection.

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Year:  1985        PMID： 3902885      PMCID： PMC268551          DOI： 10.1128/jcm.22.5.877-879.1985

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  4 in total

1.  Rapid diagnosis of human influenza infection from nasal smears by means of fluorescein-labeled antibody.

Authors:  C LIU
Journal:  Proc Soc Exp Biol Med       Date:  1956 Aug-Sep

2.  Rapid diagnosis of influenza A infection by direct immunofluorescence of nasopharyngeal aspirates in adults.

Authors:  J A Daisy; F S Lief; H M Friedman
Journal:  J Clin Microbiol       Date:  1979-06       Impact factor: 5.948

3.  Monoclonal antibodies for rapid, strain-specific identification of influenza virus isolates.

Authors:  N J Schmidt; M Ota; D Gallo; V L Fox
Journal:  J Clin Microbiol       Date:  1982-10       Impact factor: 5.948

4.  Comparison of direct immunofluorescent staining of clinical specimens for respiratory virus antigens with conventional isolation techniques.

Authors:  L Minnich; C G Ray
Journal:  J Clin Microbiol       Date:  1980-09       Impact factor: 5.948
  4 in total
  15 in total

1.  Comparison of Directigen FLU-A with viral isolation and direct immunofluorescence for the rapid detection and identification of influenza A virus.

Authors:  J L Waner; S J Todd; H Shalaby; P Murphy; L V Wall
Journal:  J Clin Microbiol       Date:  1991-03       Impact factor: 5.948

2.  Direct detection of influenza virus antigen in nasopharyngeal specimens by direct enzyme immunoassay in comparison with quantitating virus shedding.

Authors:  G Döller; W Schuy; K Y Tjhen; B Stekeler; H J Gerth
Journal:  J Clin Microbiol       Date:  1992-04       Impact factor: 5.948

Review 3.  Rapid viral diagnosis in perspective.

Authors:  P C Lee; P Hallsworth
Journal:  BMJ       Date:  1990-06-02

4.  Rapid diagnosis of influenza A and B by 24-h fluorescent focus assays.

Authors:  C E Stokes; J M Bernstein; S A Kyger; F G Hayden
Journal:  J Clin Microbiol       Date:  1988-07       Impact factor: 5.948

5.  Application of subtype-specific monoclonal antibodies for rapid detection and identification of influenza A and B viruses.

Authors:  M Ueda; A Maeda; N Nakagawa; T Kase; R Kubota; H Takakura; A Ohshima; Y Okuno
Journal:  J Clin Microbiol       Date:  1998-02       Impact factor: 5.948

6.  Rapid detection of respiratory syncytial virus and influenza A virus in cell cultures by immunoperoxidase staining with monoclonal antibodies.

Authors:  M Waris; T Ziegler; M Kivivirta; O Ruuskanen
Journal:  J Clin Microbiol       Date:  1990-06       Impact factor: 5.948

7.  Rapid detection of influenza virus by shell vial assay with monoclonal antibodies.

Authors:  M J Espy; T F Smith; M W Harmon; A P Kendal
Journal:  J Clin Microbiol       Date:  1986-10       Impact factor: 5.948

8.  Rapid determination of neutralizing antibodies to Semliki Forest virus in serum by enzyme immunoassay in cell culture with virus-specific monoclonal antibodies.

Authors:  F H van Tiel; T Harmsen; M Wagenaar; W A Boere; C A Kraaijeveld; H Snippe
Journal:  J Clin Microbiol       Date:  1986-10       Impact factor: 5.948

9.  Time-resolved fluoroimmunoassay with monoclonal antibodies for rapid diagnosis of influenza infections.

Authors:  H H Walls; K H Johansson; M W Harmon; P E Halonen; A P Kendal
Journal:  J Clin Microbiol       Date:  1986-12       Impact factor: 5.948

10.  Use of monoclonal antibodies for rapid detection of influenza A virus in nasopharyngeal secretions.

Authors:  P Pothier; G A Denoyel; S Ghim; G Prudhomme de Saint Maur; F Freymuth
Journal:  Eur J Clin Microbiol       Date:  1986-06       Impact factor: 3.267
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