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Literature DB >> 3897192

supN ochre suppressor gene in Escherichia coli codes for tRNALys.

H Uemura, S Thorbjarnardóttir, V Gamulin, J Yano, O S Andrésson, D Söll, G Eggertsson.

Abstract

We describe the cloning and nucleotide sequence of a new tRNALys gene, lysV, in Escherichia coli. An ochre suppressor allele of this gene, supN, codes for a tRNALys with anticodon UUA, presumably derived by a single base change from a wild-type UUU anticodon. The sequence of the supN tRNALys is identical to the sequence of ochre suppressor tRNAs encoded by mutant alleles at the lysT locus. This locus, which contains the two previously known tRNALys genes of E. coli, is located far from the lysV locus on the chromosome.

Entities: Chemical Gene Species

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Year: 1985 PMID： 3897192 PMCID： PMC219277 DOI： 10.1128/jb.163.3.1288-1289.1985

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

16 in total

1. Characterization and in vitro processing of transfer RNA precursors accumulated in a temperature-sensitive mutant of Escherichia coli.

Authors: H Sakano; Y Shimura
Journal: J Mol Biol Date: 1978-08-15 Impact factor: 5.469

supN ochre suppressor gene in Escherichia coli codes for tRNALys.

1. Characterization and in vitro processing of transfer RNA precursors accumulated in a temperature-sensitive mutant of Escherichia coli.

2. Gross map location of Escherichia coli transfer RNA genes.

3. Mapping of ochre suppressors in Escherichia coli.

4. Map positions and specificities of suppressor mutations in Escherichia coli K-12.

5. Lambdoid phages that simplify the recovery of in vitro recombinants.

6. Primary structure of tRNA-Lys of E. coli B.

Review 7. Linkage map of Escherichia coli K-12, edition 7.

8. A system for shotgun DNA sequencing.

9. DNA sequencing with chain-terminating inhibitors.

10. Antisuppressor mutation in Escherichia coli defective in biosynthesis of 5-methylaminomethyl-2-thiouridine.

1. Locations of genes in the 52-minute region on the physical map of Escherichia coli K-12.

2. The growth defect in Escherichia coli deficient in peptidyl-tRNA hydrolase is due to starvation for Lys-tRNA(Lys).

3. Sequence of E.coli K12 valU operon which contains 3 genes for tRNA(Val1) and 1 gene for tRNA(Lys).

Review 4. Linkage map of Escherichia coli K-12, edition 10: the traditional map.

Review 5. Linkage map of Escherichia coli K-12, edition 8.

Review 6. Transfer ribonucleic acid-mediated suppression of termination codons in Escherichia coli.

7. A Strategy for Creating Organisms Dependent on Noncanonical Amino Acids.

8. Genetic characterization of frameshift suppressors with new decoding properties.

9. tRNA hopping: enhancement by an expanded anticodon.

10. Bacteriophage T4 anticodon nuclease, polynucleotide kinase and RNA ligase reprocess the host lysine tRNA.