Literature DB >> 3893797

Specific immunoassay of alpha-amylase isoenzymes in human serum.

M Gerber, K Naujoks, H Lenz, W Gerhardt, K Wulff.   

Abstract

A monoclonal antibody (66C7) was prepared that specifically binds human salivary amylase (EC 3.2.1.1); it cross reacts with human pancreatic amylase by less than 1%. Two procedures are described for determination of isoamylases in human serum with this antibody: an enzyme immunoassay for determining amylase of salivary origin, and a routine method in which this amylase is immunoprecipitated and the remaining (pancreatic) amylase activity is assayed. Results by the two methods correlate well.

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Year:  1985        PMID: 3893797

Source DB:  PubMed          Journal:  Clin Chem        ISSN: 0009-9147            Impact factor:   8.327


  4 in total

1.  Effect of substrate size on immunoinhibition of amylase activity.

Authors:  I Warshawsky; G L Hortin
Journal:  J Clin Lab Anal       Date:  2001       Impact factor: 2.352

2.  Different patterns in immunoreactive anionic and cationic trypsinogen in urine and serum in human acute pancreatitis.

Authors:  U Petersson; S Appelros; A Borgström
Journal:  Int J Pancreatol       Date:  1999-06

3.  Parotid swelling. Does it affect serum isoamylase level?

Authors:  M Ueda; T Araki; J Kondo; K Kosaka; T Tsuji
Journal:  Dig Dis Sci       Date:  1993-07       Impact factor: 3.199

4.  Age and sex-dependent alterations of serum amylase and isoamylase levels in normal human adults.

Authors:  M Ueda; T Araki; T Shiota; K Taketa
Journal:  J Gastroenterol       Date:  1994-04       Impact factor: 7.527

  4 in total

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