Literature DB >> 3893422

Differential inhibition of two proteolytic activities in bovine lens neutral-proteinase preparations.

B J Wagner, J W Margolis, A S Abramovitz, S C Fu.   

Abstract

Hydrolysis of carbobenzoxy-Leu-Leu-Glu 2-naphthylamide by bovine lens neutral-proteinase preparations is not affected by the esterase inhibitor di-isopropyl fluorophosphate, whereas hydrolysis of carbobenzoxy-Gly-Gly-Leu p-nitroanilide is completely inhibited. Hydrolysis of alpha-crystallin, a lens structural protein, can be inhibited by only 50% after prolonged treatment with di-isopropyl fluorophosphate. These data suggest that the lens neutral-proteinase preparation contains at least two enzymes, one of which may be a serine proteinase. This may account, in part, for the previously observed complex response of the preparation to inhibitors.

Entities:  

Mesh：

Substances：

Year:  1985        PMID： 3893422      PMCID： PMC1145011          DOI： 10.1042/bj2280517

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  9 in total

1.  The colorimetric determination of leucine aminopeptidase in urine and serum of normal subjects and patients with cancer and other diseases.

Authors:  J A GOLDBARG; A M RUTENBURG
Journal:  Cancer       Date:  1958 Mar-Apr       Impact factor: 6.860

2.  Isolation and characterization of a 25K serine proteinase from bovine lens cortex.

Authors:  O P Srivastava; B J Ortwerth
Journal:  Exp Eye Res       Date:  1983-12       Impact factor: 3.467

3.  Cation-sensitive neutral endopeptidase: isolation and specificity of the bovine pituitary enzyme.

Authors:  S Wilk; M Orlowski
Journal:  J Neurochem       Date:  1980-11       Impact factor: 5.372

4.  Metal-dependent proteinase of the lens. Assay, purification and properties of the bovine enzyme.

Authors:  A M Blow; R V Heyningen; A J Barrett
Journal:  Biochem J       Date:  1975-03       Impact factor: 3.857

5.  Experimental studies on cataract.

Authors:  R van Heyningen
Journal:  Invest Ophthalmol Vis Sci       Date:  1976-09       Impact factor: 4.799

6.  A synthetic endopeptidase substrate hydrolyzed by the bovine lens neutral proteinase preparation.

Authors:  B J Wagner; S C Fu; J W Margolis; K R Fleshman
Journal:  Exp Eye Res       Date:  1984-05       Impact factor: 3.467

7.  Limited proteolysis of bovine lens alpha-crystallin by calpain, a Ca2+-dependent cysteine proteinase, isolated from the same tissue.

Authors:  H Yoshida; T Murachi; I Tsukahara
Journal:  Biochim Biophys Acta       Date:  1984-04-10

8.  Calf lens neutral proteinase activity using calf lens crystallin substrates.

Authors:  B J Wagner; J W Margolis; P N Farnsworth; S C Fu
Journal:  Exp Eye Res       Date:  1982-09       Impact factor: 3.467

9.  Presence of low molecular weight polypeptides in human brunescent cataracts.

Authors:  J Horwitz; J S Hansen; C C Cheung; L L Ding; B R Straatsma; D O Lightfoot; L J Takemoto
Journal:  Biochem Biophys Res Commun       Date:  1983-05-31       Impact factor: 3.575
  9 in total
  3 in total

Review 1.  Common cell biologic and biochemical changes in aging and age-related diseases of the eye: toward new therapeutic approaches to age-related ocular diseases.

Authors:  Elizabeth A Whitcomb; Fu Shang; Allen Taylor
Journal:  Invest Ophthalmol Vis Sci       Date:  2013-12-13       Impact factor: 4.799

2.  Common epitopes of bovine lens multicatalytic-proteinase-complex subunits.

Authors:  B J Wagner; J W Margolis
Journal:  Biochem J       Date:  1989-01-01       Impact factor: 3.857

Review 3.  Ultrasound of acquired posterior fossa abnormalities in the newborn.

Authors:  Monica Fumagalli; Alessandro Parodi; Luca Ramenghi; Catherine Limperopoulos; Sylke Steggerda
Journal:  Pediatr Res       Date:  2020-03       Impact factor: 3.756
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.