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Literature DB >> 3891694

Detection of glucagon in pancreatic A-cells by monoclonal antibodies.

Abstract

The production of a mouse monoclonal antibody from a hybrid myeloma and its use for the detection of glucagon in tissue sections is reported. The hybrid clone isolated after fusion of mouse myeloma cells with hyperimmune spleen cells from a mouse previously immunized with porcine glucagon allowed us a standardized and permanent source of monoclonal antibodies in a culture cell system. The monoclonal antibody (3 GL 31) specifically reacts with pancreatic A-cells in several species including pig, rabbit, tupaia belangeri and sheep. No immunoreactivity is observed against gut cells and neurons.

Entities: Disease Species

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Year: 1985 PMID： 3891694 DOI： 10.1007/bf00494065

Source DB: PubMed Journal: Histochemistry ISSN： 0301-5564

22 in total

10. Isolation and characterization of the intestinal peptide porcine PHI (PHI-27), a new member of the glucagon--secretin family.

Authors: K Tatemoto; V Mutt
Journal: Proc Natl Acad Sci U S A Date: 1981-11 Impact factor: 11.205

2 in total

1. Monoclonal antibodies against calcitonin. Characterization and application in light and electron microscopy immunocytochemistry.

Authors: L Scopsi; J Arias; G Houen; G Racchetti; G L Fossati; Y M Galante
Journal: Histochemistry Date: 1988

2. Immunohistochemical localization of glucagon-like peptide 1. Use of poly- and monoclonal antibodies.

Authors: T Kauth; J Metz
Journal: Histochemistry Date: 1987

2 in total

Detection of glucagon in pancreatic A-cells by monoclonal antibodies.

1. Is GIP a glucagon cell constituent?

2. Localization of glucagon in the alpha cells in the pancreatic islet by immunofluorescent technics.

3. Species variations in the primary structure of glucagon.

4. Detection of substance P in the central nervous system by a monoclonal antibody.

5. Isolation of a glucagon-containing peptide: primary structure of a possible fragment of proglucagon.

6. A gastric inhibitory polypeptide. I. The amino acid composition and the tryptic peptides.

7. Evidence of sequential metabolic cleavage of proglucagon to glucagon in glucagon biosynthesis.

8. Glucagon and glicentin immunoreactive cells in human colon.

9. Mammalian pancreatic preproglucagon contains three glucagon-related peptides.

10. Isolation and characterization of the intestinal peptide porcine PHI (PHI-27), a new member of the glucagon--secretin family.

1. Monoclonal antibodies against calcitonin. Characterization and application in light and electron microscopy immunocytochemistry.

2. Immunohistochemical localization of glucagon-like peptide 1. Use of poly- and monoclonal antibodies.