Literature DB >> 3889844

Optimizing the expression in E. coli of a synthetic gene encoding somatomedin-C (IGF-I).

G Buell, M F Schulz, G Selzer, A Chollet, N R Movva, D Semon, S Escanez, E Kawashima.   

Abstract

Double-stranded DNA encoding the human hormone somatomedin-C (SMC) has been synthesized. This synthetic gene has been inserted into a plasmid bearing the strong leftward promoter (PL) of bacteriophage lambda and expressed in E. coli. Codons for the N-terminal region of SMC which maximized the hormone's synthesis were chosen in an SMC-lac z fusion assay. The amounts of SMC accumulated in E. coli were influenced by mutations at two chromosomal loci, lon and htpR.

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Year:  1985        PMID： 3889844      PMCID： PMC341125          DOI： 10.1093/nar/13.6.1923

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  31 in total

1.  Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis.

Authors:  T Maniatis; A Jeffrey; H van deSande
Journal:  Biochemistry       Date:  1975-08-26       Impact factor: 3.162

2.  Isolation of recombinant plasmids bearing cDNA to hen ovomucoid and lysozyme mRNAs.

Authors:  G N Buell; M P Wickens; J Carbon; R T Schimke
Journal:  J Biol Chem       Date:  1979-09-25       Impact factor: 5.157

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

4.  A temperature sensitive nonsense mutation affecting the synthesis of a major protein of Escherichia coli K12.

Authors:  S Cooper; T Ruettinger
Journal:  Mol Gen Genet       Date:  1975-08-05

5.  Sequencing end-labeled DNA with base-specific chemical cleavages.

Authors:  A M Maxam; W Gilbert
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

6.  Insulin-like growth factor I stimulates growth in hypophysectomized rats.

Authors:  E Schoenle; J Zapf; R E Humbel; E R Froesch
Journal:  Nature       Date:  1982-03-18       Impact factor: 49.962

7.  Chemical synthesis of genes for human insulin.

Authors:  R Crea; A Kraszewski; T Hirose; K Itakura
Journal:  Proc Natl Acad Sci U S A       Date:  1978-12       Impact factor: 11.205

8.  Correlation between the abundance of Escherichia coli transfer RNAs and the occurrence of the respective codons in its protein genes: a proposal for a synonymous codon choice that is optimal for the E. coli translational system.

Authors:  T Ikemura
Journal:  J Mol Biol       Date:  1981-09-25       Impact factor: 5.469

9.  Genetic control of heat-shock protein synthesis and its bearing on growth and thermal resistance in Escherichia coli K-12.

Authors:  T Yamamori; T Yura
Journal:  Proc Natl Acad Sci U S A       Date:  1982-02       Impact factor: 11.205

10.  The amino acid sequence of human insulin-like growth factor I and its structural homology with proinsulin.

Authors:  E Rinderknecht; R E Humbel
Journal:  J Biol Chem       Date:  1978-04-25       Impact factor: 5.157
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  26 in total

1.  Secondary structure of the ribosome binding site determines translational efficiency: a quantitative analysis.

Authors:  M H de Smit; J van Duin
Journal:  Proc Natl Acad Sci U S A       Date:  1990-10       Impact factor: 11.205

Review 2.  High-expression of a target gene and high-stability of the plasmid.

Authors:  M Kobayashi; Y Kurusu; H Yukawa
Journal:  Appl Biochem Biotechnol       Date:  1991-02       Impact factor: 2.926

3.  Translational reinitiation in the presence and absence of a Shine and Dalgarno sequence.

Authors:  R A Spanjaard; J van Duin
Journal:  Nucleic Acids Res       Date:  1989-07-25       Impact factor: 16.971

4.  Increased production of low molecular weight recombinant proteins in Escherichia coli.

Authors:  R M Belagaje; S G Reams; S C Ly; W F Prouty
Journal:  Protein Sci       Date:  1997-09       Impact factor: 6.725

5.  Use of synthetic ribosome binding site for overproduction of the 5B protein of insertion sequence IS5.

Authors:  J M Chernak; H O Smith
Journal:  Nucleic Acids Res       Date:  1989-03-11       Impact factor: 16.971

6.  Detection of common motifs in RNA secondary structures.

Authors:  H Margalit; B A Shapiro; A B Oppenheim; J V Maizel
Journal:  Nucleic Acids Res       Date:  1989-06-26       Impact factor: 16.971

7.  Use of Bacillus brevis for efficient synthesis and secretion of human epidermal growth factor.

Authors:  H Yamagata; K Nakahama; Y Suzuki; A Kakinuma; N Tsukagoshi; S Udaka
Journal:  Proc Natl Acad Sci U S A       Date:  1989-05       Impact factor: 11.205

Review 8.  Strategies for achieving high-level expression of genes in Escherichia coli.

Authors:  S C Makrides
Journal:  Microbiol Rev       Date:  1996-09

9.  Characterization of three putative Lon proteases of Thermus thermophilus HB27 and use of their defective mutants as hosts for production of heterologous proteins.

Authors:  Tomoko Maehara; Takayuki Hoshino; Akira Nakamura
Journal:  Extremophiles       Date:  2007-12-22       Impact factor: 2.395

10.  Secondary structure as primary determinant of the efficiency of ribosomal binding sites in Escherichia coli.

Authors:  A C Looman; J Bodlaender; M de Gruyter; A Vogelaar; P H van Knippenberg
Journal:  Nucleic Acids Res       Date:  1986-07-11       Impact factor: 16.971
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