Literature DB >> 3885846

Cloning and expression of Staphylococcus aureus plasmid-mediated quaternary ammonium resistance in Escherichia coli.

J M Tennent, B R Lyon, M T Gillespie, J W May, R A Skurray.   

Abstract

The Staphylococcus aureus plasmid pSK1 carries Tn4001, a 4.7-kilobase (kb) transposon which specifies resistance to gentamicin, tobramycin, and kanamycin. In addition, pSK1 mediates resistance to trimethoprim and linked resistance to ethidium bromide (Ebr) and to quaternary ammonium compounds (Qar). Restriction endonuclease analysis of pSK1 and a deleted derivative of pSK1 revealed that the gene(s) responsible for Ebr Qar lies within a 5.2-kb HindIII fragment. This fragment has been cloned into the Escherichia coli plasmid vector pBR322, and transformants of an E. coli K-12 strain exhibited Ebr Qar. Subcloning of the 5.2-kb insert, combined with data from electron microscopic analysis of deleted derivatives of pSK1, located the Ebr Qar determinant(s) on a 2.3-kb segment of pSK1 DNA.

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Year:  1985        PMID： 3885846      PMCID： PMC176209          DOI： 10.1128/AAC.27.1.79

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  14 in total

1.  Tn4001: a gentamicin and kanamycin resistance transposon in Staphylococcus aureus.

Authors:  B R Lyon; J W May; R A Skurray
Journal:  Mol Gen Genet       Date:  1984

2.  Analysis of plasmids in nosocomial strains of multiple-antibiotic-resistant Staphylococcus aureus.

Authors:  B R Lyon; J W May; R A Skurray
Journal:  Antimicrob Agents Chemother       Date:  1983-06       Impact factor: 5.191

3.  Cloning and polypeptide analysis of the leading region in F plasmid DNA transfer.

Authors:  A Ray; R Skurray
Journal:  Plasmid       Date:  1983-05       Impact factor: 3.466

4.  Plasmid-mediated resistance to quaternary ammonium compounds in methicillin-resistant Staphylococcus aureus.

Authors:  D E Townsend; L Greed; N Ashdown; W B Grubb
Journal:  Med J Aust       Date:  1983-10-01       Impact factor: 7.738

5.  Ethidium bromide resistance, a new marker on the staphylococcal penicillinase plasmid.

Authors:  L H Johnston; K G Dyke
Journal:  J Bacteriol       Date:  1969-12       Impact factor: 3.490

6.  Self-transmissible plasmids in staphylococci that encode resistance to aminoglycosides.

Authors:  G L Archer; J L Johnston
Journal:  Antimicrob Agents Chemother       Date:  1983-07       Impact factor: 5.191

7.  Molecular epidemiology of multiresistant Staphylococcus aureus in Australian hospitals.

Authors:  B R Lyon; J L Iuorio; J W May; R A Skurray
Journal:  J Med Microbiol       Date:  1984-02       Impact factor: 2.472

8.  Gentamicin resistance in methicillin-resistant Staphylococcus aureus.

Authors:  D E Townsend; W B Grubb; N Ashdown
Journal:  Pathology       Date:  1983-04       Impact factor: 5.306

9.  Conjugational transfer of gentamicin resistance plasmids intra- and interspecifically in Staphylococcus aureus and Staphylococcus epidermidis.

Authors:  R W McDonnell; H M Sweeney; S Cohen
Journal:  Antimicrob Agents Chemother       Date:  1983-01       Impact factor: 5.191

10.  Two restriction and modification systems in Staphylococcus aureus NCTC8325.

Authors:  S Iordanescu; M Surdeanu
Journal:  J Gen Microbiol       Date:  1976-10
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  32 in total

Review 1.  Potential impact of increased use of biocides in consumer products on prevalence of antibiotic resistance.

Authors:  Peter Gilbert; Andrew J McBain
Journal:  Clin Microbiol Rev       Date:  2003-04       Impact factor: 26.132

Review 2.  Transport systems encoded by bacterial plasmids.

Authors:  L S Tisa; B P Rosen
Journal:  J Bioenerg Biomembr       Date:  1990-08       Impact factor: 2.945

Review 3.  The TetR family of transcriptional repressors.

Authors:  Juan L Ramos; Manuel Martínez-Bueno; Antonio J Molina-Henares; Wilson Terán; Kazuya Watanabe; Xiaodong Zhang; María Trinidad Gallegos; Richard Brennan; Raquel Tobes
Journal:  Microbiol Mol Biol Rev       Date:  2005-06       Impact factor: 11.056

4.  Complete nucleotide sequence of pSK41: evolution of staphylococcal conjugative multiresistance plasmids.

Authors:  T Berg; N Firth; S Apisiridej; A Hettiaratchi; A Leelaporn; R A Skurray
Journal:  J Bacteriol       Date:  1998-09       Impact factor: 3.490

5.  Selection of multiple-antibiotic-resistant (mar) mutants of Escherichia coli by using the disinfectant pine oil: roles of the mar and acrAB loci.

Authors:  M C Moken; L M McMurry; S B Levy
Journal:  Antimicrob Agents Chemother       Date:  1997-12       Impact factor: 5.191

Review 6.  Proton-dependent multidrug efflux systems.

Authors:  I T Paulsen; M H Brown; R A Skurray
Journal:  Microbiol Rev       Date:  1996-12

Review 7.  Antimicrobial resistance of Staphylococcus aureus: genetic basis.

Authors:  B R Lyon; R Skurray
Journal:  Microbiol Rev       Date:  1987-03

8.  The 3' conserved segment of integrons contains a gene associated with multidrug resistance to antiseptics and disinfectants.

Authors:  I T Paulsen; T G Littlejohn; P Rådström; L Sundström; O Sköld; G Swedberg; R A Skurray
Journal:  Antimicrob Agents Chemother       Date:  1993-04       Impact factor: 5.191

9.  Molecular characterization of the staphylococcal multidrug resistance export protein QacC.

Authors:  I T Paulsen; M H Brown; S J Dunstan; R A Skurray
Journal:  J Bacteriol       Date:  1995-05       Impact factor: 3.490

Review 10.  Chlorhexidine: antibacterial action and bacterial resistance.

Authors:  A D Russell
Journal:  Infection       Date:  1986 Sep-Oct       Impact factor: 3.553
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