Literature DB >> 3881474

Enzyme immunoassay for evaluation of Toxoplasma gondii growth in tissue culture.

A Merli, A Canessa, G Melioli.   

Abstract

An enzyme-linked immunosorbent assay (ELISA) for the evaluation of growth kinetics of Toxoplasma gondii in tissue cultures was developed. Tissue culture microplates (96 wells) were seeded with Vero cells, infected with a virulent strain of T. gondii, and incubated for different time periods. The ELISA was performed with anti-T. gondii antibodies on the infected cells. The method was simple, rapid, and accurate, and very good correlations between the ELISA results and the percentage of T. gondii rosettes in infected cells, the number of free T. gondii in the supernatant, and the amount of T. gondii antigen in the supernatant were observed.

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Year:  1985        PMID： 3881474      PMCID： PMC271581          DOI： 10.1128/jcm.21.1.88-91.1985

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  5 in total

1.  The effect of trimethoprim and sulfamethoxazole on Toxoplasma gondii in vitro and in vivo.

Authors:  P L Grossman; J S Remington
Journal:  Am J Trop Med Hyg       Date:  1979-05       Impact factor: 2.345

2.  A method to evaluate the capacity of monocytes and macrophages to inhibit multiplication of an intracellular pathogen.

Authors:  R McLeod; J S Remington
Journal:  J Immunol Methods       Date:  1979-05-10       Impact factor: 2.303

3.  In vitro lymphocyte stimulation with specific antigen in congenital toxoplasmosis.

Authors:  O Closs; B Stray-Pedersen
Journal:  Scand J Infect Dis       Date:  1981

4.  Rapid solid-phase enzyme immunoassay for antibodies to viruses and other microbes: effects of polyethylene glycol.

Authors:  E M Salonen; A Vaheri
Journal:  J Immunol Methods       Date:  1981       Impact factor: 2.303

5.  The interaction between Toxoplasma gondii and mammalian cells. I. Mechanism of entry and intracellular fate of the parasite.

Authors:  T C Jones; S Yeh; J G Hirsch
Journal:  J Exp Med       Date:  1972-11-01       Impact factor: 14.307
  5 in total
  5 in total

1.  High-throughput growth assay for Toxoplasma gondii using yellow fluorescent protein.

Authors:  Marc-Jan Gubbels; Catherine Li; Boris Striepen
Journal:  Antimicrob Agents Chemother       Date:  2003-01       Impact factor: 5.191

2.  In situ enzyme-linked immunosorbent assay to quantitate in vitro development of Eimeria tenella.

Authors:  J A Olson
Journal:  Antimicrob Agents Chemother       Date:  1990-07       Impact factor: 5.191

3.  Selective labeling of intracellular parasite proteins by using ricin.

Authors:  A M Gurnett; P M Dulski; S J Darkin-Rattray; M J Carrington; D M Schmatz
Journal:  Proc Natl Acad Sci U S A       Date:  1995-03-14       Impact factor: 11.205

4.  Enzyme immunoassay to assess effect of antimicrobial agents on Toxoplasma gondii in tissue culture.

Authors:  F Derouin; C Chastang
Journal:  Antimicrob Agents Chemother       Date:  1988-03       Impact factor: 5.191

5.  In Vitro Effects of Ivermectin and Sulphadiazine on Toxoplasma gondii.

Authors:  Melek Bilgin; Tuba Yıldırım; Murat Hökelek
Journal:  Balkan Med J       Date:  2013-03-01       Impact factor: 2.021
  5 in total

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