Melek Bilgin1, Tuba Yıldırım2, Murat Hökelek3. 1. Microbiology Laboratory, Samsun Education and Research Hospital, Samsun, Turkey. 2. Department of Biology, Faculty of Arts and Sciences, Amasya University, Amasya, Turkey. 3. Department of Medical Microbiology, Cerrahpaşa Faculty of Medicine, İstanbul University, İstanbul, Turkey.
Abstract
OBJECTIVE: Ivermectin and sulphadiazine were tested individually to determine their in vitro effects on Toxoplasma gondii grown in human epidermoid larynx carcinoma (Hep-2) cell culture. STUDY DESIGN: In-vitro study. MATERIAL AND METHODS: Toxoplasma growth was quantities by an enzyme immunoassay performed directly on the fixed cultures, using a rabbit anti-T. gondii immunoglobulin G as the first antibody and a phosphatase-labeled anti-rabbit immunoglobulin G as the second antibody. For each drug, regression models were used to quantify the relationship between optical density values and antimicrobial agent concentrations in the cultures. RESULTS: The 50% inhibitory concentrations (IC50) of ivermectin and sulphadiazine were found to be 0.2 μg/mL and 7.3 μg/mL after 48 h of exposure, respectively. None of the concentrations tested for each drugs demonstrated toxicity to Hep-2 cells after 72 h of incubation. CONCLUSION: These results indicate that ivermectin significantly inhibited replication of the tachyzoites of T. gondii RH strain.
OBJECTIVE:Ivermectin and sulphadiazine were tested individually to determine their in vitro effects on Toxoplasma gondii grown in humanepidermoid larynx carcinoma (Hep-2) cell culture. STUDY DESIGN: In-vitro study. MATERIAL AND METHODS:Toxoplasma growth was quantities by an enzyme immunoassay performed directly on the fixed cultures, using a rabbit anti-T. gondii immunoglobulin G as the first antibody and a phosphatase-labeled anti-rabbit immunoglobulin G as the second antibody. For each drug, regression models were used to quantify the relationship between optical density values and antimicrobial agent concentrations in the cultures. RESULTS: The 50% inhibitory concentrations (IC50) of ivermectin and sulphadiazine were found to be 0.2 μg/mL and 7.3 μg/mL after 48 h of exposure, respectively. None of the concentrations tested for each drugs demonstrated toxicity to Hep-2 cells after 72 h of incubation. CONCLUSION: These results indicate that ivermectin significantly inhibited replication of the tachyzoites of T. gondii RH strain.
Entities:
Keywords:
Hep-2; Toxoplasma gondii; in vitro; ivermectin; sulphadiazine
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