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Literature DB >> 3881410

Expression of a Saccharomyces cerevisiae photolyase gene in Escherichia coli.

Abstract

A 3.3-kilobase PvuII fragment carrying the PHR1 gene of Saccharomyces cerevisiae has been cloned into an Escherichia coli expression vector and introduced into E. coli strains deficient in DNA photolyase. Complementation of the E. coli phr-1 mutation was observed, strongly suggesting that the yeast PHR1 gene encodes a DNA photolyase.

Entities: Gene Species

Mesh：

Substances：

Year: 1985 PMID： 3881410 PMCID： PMC214950 DOI： 10.1128/jb.161.2.769-771.1985

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

17 in total

1. Analysis of photoenzymatic repair of UV lesions in DNA by single light flashes. II. In vivo studies with Escherichia coli cells and bacteriophage.

Authors: W Harm; H Harm; C S Rupert
Journal: Mutat Res Date: 1968 Nov-Dec Impact factor: 2.433

2. Spectral properties of the chromophoric material associated with the deoxyribonucleic acid photoreactivating enzyme isolated from baker's yeast.

Authors: S Minato; H Werbin
Journal: Biochemistry Date: 1971-11-23 Impact factor: 3.162

3. Functional genetic expression of eukaryotic DNA in Escherichia coli.

Authors: K Struhl; J R Cameron; R W Davis
Journal: Proc Natl Acad Sci U S A Date: 1976-05 Impact factor: 11.205

4. Purification of Escherichia coli DNA photolyase.

Authors: A Sancar; F W Smith; G B Sancar
Journal: J Biol Chem Date: 1984-05-10 Impact factor: 5.157

5. Identification of a neutral flavin radical and characterization of a second chromophore in Escherichia coli DNA photolyase.

Authors: M S Jorns; G B Sancar; A Sancar
Journal: Biochemistry Date: 1984-06-05 Impact factor: 3.162

5. Interactions between yeast photolyase and nucleotide excision repair proteins in Saccharomyces cerevisiae and Escherichia coli.

Authors: G B Sancar; F W Smith
Journal: Mol Cell Biol Date: 1989-11 Impact factor: 4.272

6. Enhanced UV resistance and improved killing of malaria mosquitoes by photolyase transgenic entomopathogenic fungi.

Authors: Weiguo Fang; Raymond J St Leger
Journal: PLoS One Date: 2012-08-17 Impact factor: 3.240

6 in total

Expression of a Saccharomyces cerevisiae photolyase gene in Escherichia coli.

1. Analysis of photoenzymatic repair of UV lesions in DNA by single light flashes. II. In vivo studies with Escherichia coli cells and bacteriophage.

2. Spectral properties of the chromophoric material associated with the deoxyribonucleic acid photoreactivating enzyme isolated from baker's yeast.

3. Functional genetic expression of eukaryotic DNA in Escherichia coli.

4. Purification of Escherichia coli DNA photolyase.

5. Identification of a neutral flavin radical and characterization of a second chromophore in Escherichia coli DNA photolyase.

6. Evidence that deoxyribonucleic acid photolyase from baker's yeast is a flavoprotein.

7. Cloning and mapping of Saccharomyces cerevisiae photoreactivation gene PHR1.

8. Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli.

9. Escherichia coli DNA photolyase is a flavoprotein.

10. Identification and amplification of the E. coli phr gene product.

1. Photoreactivation of UV-induced cyclobutane pyrimidine dimers in the MFA2 gene of Saccharomyces cerevisiae.

2. Expression of the yeast PHR1 gene is induced by DNA-damaging agents.

3. Sequence of the Saccharomyces cerevisiae PHR1 gene and homology of the PHR1 photolyase to E. coli photolyase.

Review 4. Deoxyribonucleic acid repair in the yeast Saccharomyces cerevisiae.

5. Interactions between yeast photolyase and nucleotide excision repair proteins in Saccharomyces cerevisiae and Escherichia coli.

6. Enhanced UV resistance and improved killing of malaria mosquitoes by photolyase transgenic entomopathogenic fungi.