Literature DB >> 387087

Two forms of pyruvate kinase in Escherichia coli. A comparison of chemical and molecular properties.

G Valentini, P Iadarola, B L Somani, M Malcovati.   

Abstract

The two forms of pyruvate kinase (ATP:pyruvate 2-O-phosphotransferase, EC 2.7.1.40) present in Escherichia coli have been purified from the same cultures and crystallized. A modified procedure for the purification of type I pyruvate kinase is described. Molecular weight, subunit structure, amino acid composition, NH2-terminal amino acid, maps of tryptic peptides and conditions for crystallization have been determined for the two forms. A comparison of these data shows that the two forms are different proteins, each being a tetramer of identical subunits.

Entities:  

Mesh：

Substances：

Year:  1979        PMID： 387087     DOI： 10.1016/0005-2744(79)90145-1

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  12 in total

1.  Structure of the Aspergillus nidulans pyruvate kinase gene.

Authors:  L de Graaff; J Visser
Journal:  Curr Genet       Date:  1988-12       Impact factor: 3.886

2.  Purification and kinetic properties of pyruvate kinase isoenzymes of Salmonella typhimurium.

Authors:  C Garcia-Olalla; A Garrido-Pertierra
Journal:  Biochem J       Date:  1987-01-15       Impact factor: 3.857

3.  Direct genomic sequencing of bacterial DNA: the pyruvate kinase I gene of Escherichia coli.

Authors:  O Ohara; R L Dorit; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1989-09       Impact factor: 11.205

4.  Flexible Metabolism and Suppression of Latent Enzymes Are Important for Escherichia coli Adaptation to Diverse Environments within the Host.

Authors:  Christopher J Alteri; Stephanie D Himpsl; Allyson E Shea; Harry L T Mobley
Journal:  J Bacteriol       Date:  2019-07-24       Impact factor: 3.490

5.  Isolation and properties of the glycolytic enzymes from Zymomonas mobilis. The five enzymes from glyceraldehyde-3-phosphate dehydrogenase through to pyruvate kinase.

Authors:  A Pawluk; R K Scopes; K Griffiths-Smith
Journal:  Biochem J       Date:  1986-08-15       Impact factor: 3.857

6.  Enzymes of glucose and methanol metabolism in the actinomycete Amycolatopsis methanolica.

Authors:  A M Alves; G J Euverink; H J Hektor; G I Hessels; J van der Vlag; J W Vrijbloed; D Hondmann; J Visser; L Dijkhuizen
Journal:  J Bacteriol       Date:  1994-11       Impact factor: 3.490

7.  Purification and properties of two isozymes of pyruvate kinase from Mucor racemosus.

Authors:  T M Hohn; J L Paznokas
Journal:  J Bacteriol       Date:  1987-08       Impact factor: 3.490

8.  cDNA cloning of human R-type pyruvate kinase and identification of a single amino acid substitution (Thr384----Met) affecting enzymatic stability in a pyruvate kinase variant (PK Tokyo) associated with hereditary hemolytic anemia.

Authors:  H Kanno; H Fujii; A Hirono; S Miwa
Journal:  Proc Natl Acad Sci U S A       Date:  1991-09-15       Impact factor: 11.205

Review 9.  Pyruvate kinase: activation by and catalytic role of the monovalent and divalent cations.

Authors:  T Nowak; C Suelter
Journal:  Mol Cell Biochem       Date:  1981-03-13       Impact factor: 3.396

10.  Engineering Escherichia coli to increase triacetic acid lactone (TAL) production using an optimized TAL sensor-reporter system.

Authors:  Ye Li; Shuai Qian; Rachel Dunn; Patrick C Cirino
Journal:  J Ind Microbiol Biotechnol       Date:  2018-07-25       Impact factor: 3.346
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.